| 番薯过氧化物酶的分离纯化及其性质研究 |
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| 引用本文： | 李霞,韩凤,何睿,焦艳华,梁媛媛,陈灿玉,谢恬. 番薯过氧化物酶的分离纯化及其性质研究[J]. 杭州师范大学学报(自然科学版), 2011, 0(3): 253-257 |
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| 作者姓名： | 李霞 韩凤 何睿 焦艳华 梁媛媛 陈灿玉 谢恬 |
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| 作者单位： | 杭州师范大学生物医药与健康研究中心; |
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| 基金项目： | 浙江省卫生厅科技计划项目(2009B124) |
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| 摘 要： | 从番薯皮中分离和纯化番薯过氧化物酶,对其酶学性质进行表征,分析了温度、pH对酶促反应的影响,考查了酶的热稳定性、酸碱稳定性.分离纯化过程包括匀浆、水提、双水相萃取、Sepharose CL-6B凝胶层析、Phenyl Sepharose 6 fast flow疏水层析、Con A Sepharose 4B亲和层析.经纯...
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| 关 键 词： | 番薯 过氧化物酶 纯化与性质 |
Purification and Characterization of Sweet Potato Peroxidase |
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| LI Xia,HAN Feng,HE Rui,JIAO Yan-hua,LIANG Yuan-yuan,CHEN Can-yu,XIE Tian. Purification and Characterization of Sweet Potato Peroxidase[J]. , 2011, 0(3): 253-257 |
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| Authors: | LI Xia HAN Feng HE Rui JIAO Yan-hua LIANG Yuan-yuan CHEN Can-yu XIE Tian |
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| Affiliation: | LI Xia,HAN Feng,HE Rui,JIAO Yan-hua,LIANG Yuan-yuan,CHEN Can-yu,XIE Tian(Research Center of Biomedicine and Health,Hangzhou Normal University,Hangzhou 310012,China) |
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| Abstract: | The experiment purified and characterized the sweet potato peroxidase from sweet potato peel of Ipomoea batatas.The extraction and purification procedure included homogenate,water extraction,aqueous two-phase extraction,Sepharose CL-6B gel filtration,Phenyl Sepharose 6 fast flow hydrophobic chromatography and Con A Sepharose 4B affinity chromatography.The specific activity of purified peroxidase was 6 930 U/mg.The molecular weigh of sweet potato peroxidase was estimated to be 34 000 by SDS-PAGE as a single ... |
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| Keywords: | Ipomoea batatas peroxidase purification and characterization |
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