Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles |
| |
| Authors: | B Sarkadi A Schubert G Gárdos |
| |
| Institution: | (1) National Institute of Haematology and Blood Transfusion, Budapest;(2) Department of Physics, University of Agricultural Sciences, Gödöllö, Hungary |
| |
| Abstract: | Summary In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.Acknowledgments. This work was supported by the Scientific Research Council, Ministry of Health, Hungary (6-03-0306-01-1/Gá). We thank for the valuable comments of Dr Ilma Szász and for the skilful technical assistance of Mrs M. Sarkadi. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
