| 云芝多糖在NO诱导的巨噬细胞凋亡中的分子基础 |
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| 引用本文： | 王瑾雯,陈瑗,王章. 云芝多糖在NO诱导的巨噬细胞凋亡中的分子基础[J]. 中山大学学报(自然科学版), 2001, 40(2): 68-71 |
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| 作者姓名： | 王瑾雯 陈瑗 王章 |
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| 作者单位： | 1. 中山大学
2. 第一军医大学生化教研室, |
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| 基金项目： | 国家自然科学基金资助项目（39570867） |
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| 摘 要： | 在应用云芝多糖的基础上，用NO诱导居噬细胞凋亡，发现云芝多糖可加强NO诱导的细胞凋亡；通过RT－PCR观察诱导生性一氧化氮合酶基因的表达情况，结果表明云芝多糖能诱导一氧化氮合酶基因表达，并可增强γ干扰纱和脂多糖的诱导作用，提示云芝多糖在动脉粥样硬化中的防治作用是通过诱导诱生性一氧化氮合酶表达而实现的。
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| 关 键 词： | 云芝多糖 细胞凋亡 诱生性－氧化氮合酶 动脉粥样硬化 巨噬细胞 诱导 分子基础 |
| 文章编号： | 0529-6579(2001)02-0068-04 |
| 修稿时间： | 2000-05-26 |
Molecular Mechanism of Effects of PSK on the Apoptosis of Macrophages Induced by NO |
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| WANG Jin-wen,CHEN Yuan,WANG Xun-zhang. Molecular Mechanism of Effects of PSK on the Apoptosis of Macrophages Induced by NO[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2001, 40(2): 68-71 |
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| Authors: | WANG Jin-wen CHEN Yuan WANG Xun-zhang |
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| Affiliation: | WANG Jin wen 1,CHEN Yuan 2,WANG Xun zhang 1 |
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| Abstract: | To investigate the molecular mechanism of polysaccharide krestin (PSK) on atherosclerosis's prevention and treatment.Macrophages were cultured and treated with or without PSK initially, Macrophage apoptosis was induced by NO and oxidized low-density lipoprotein(ox-LDL), Expression of inducible nitric oxide synthase(iNOS) mRNA by Raw264.7 macrophages as assessed by RT-PCR.PSK can inhibit ox-LDL-induced macrophage apoptosis and promote NO-induced macrophages apoptosis; Raw264.7 macrophages can be induced to express iNOS mRNA when stimulated with PSK, IFN-γ and LPS in vitro, PSK can enhance the effects of IFN-γ and LPS,suppress the inhibition effects of ox-LDL.The prevention and treatment effects of PSK on atherosclerosis may be realized by inducing iNOS. |
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| Keywords: | polysaccharide krestin apoptosis inducible nitric oxide synthase atherosclerosis |
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