| A pair of two－component regulatory genes ecrA1／A2 in S. coelicolor |
| |
| 引用本文： | Li YQ,Chen PL,Chen SF,Wu D,Zheng J. A pair of two－component regulatory genes ecrA1／A2 in S. coelicolor[J]. 浙江大学学报(自然科学英文版), 2004, 5(2): 173-179 |
| |
| 作者姓名： | Li YQ Chen PL Chen SF Wu D Zheng J |
| |
| 作者单位： | [1]CollegeofLifeScience [2]InstituteofBioengineering,ZhejiangUniversity,Hongzhou310027,China [3]DrugAdiministrationofZhejiangProvince,Hongzhou310012,China |
| |
| 摘 要： | Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed that ecrA1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway, ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA 1/A2 was positive regulatory element for red gene cluster.
|
| 关 键 词： | 调节基因 基因簇 抗生素 DNA 脱氧核糖核酸 |
A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor |
| |
| Li Yong-quan,Chen Pei-lin,Chen Shi-fei,Wu Dan,Zheng Jing. A pair of two-component regulatory genes ecrA1/A2 in S. coelicolor[J]. Journal of Zhejiang University Science, 2004, 5(2): 173-179 |
| |
| Authors: | Li Yong-quan Chen Pei-lin Chen Shi-fei Wu Dan Zheng Jing |
| |
| Affiliation: | College of Life Science, Zhejiang University, Hongzhou 310027, China. lyq@m.hz.zj.cn |
| |
| Abstract: | Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor. DNA microarray analysis revealed that ecrA1/A2, which mapped at distant sites from red locus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecrA1 and ecrA2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecrA1/A2 was positive regulatory element for red gene cluster. |
| |
| Keywords: | |
| 本文献已被 维普 PubMed 等数据库收录！ |
|
