| 应用单细胞凝胶电泳技术测定农药对蚯蚓的DNA损伤 |
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| 引用本文： | 封少龙,罗屿,钟远,谢朝云,孔志明. 应用单细胞凝胶电泳技术测定农药对蚯蚓的DNA损伤[J]. 南京大学学报(自然科学版), 2000, 36(5): 649-652 |
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| 作者姓名： | 封少龙 罗屿 钟远 谢朝云 孔志明 |
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| 作者单位： | [1]污染控制与资源化研究国家重点实验室,南京大学环境科学与 [2]污染控制与资源化研究国家重点实验室,南京大学环境 |
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| 基金项目： | 国家自然科学基金!(396 70 15 3) |
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| 摘 要： | 探讨采用当前国际上较先进的ＤＮＡ损伤检测技术－单细胞凝胶电泳技术，来检测农药对蚯蚓ＤＮＡ的损伤，由于蚯蚓是陆生生物与土壤生物之间传递污染物的桥梁，能很好地指示土壤环境的变化，是评价农药对生态环境安全性的一项重要指标。尝试建立蚯蚓的单细胞凝胶电泳来评价农药对生态环境的影响，结果表明两种新型农药对蚯蚓的ＤＮＡ均有损伤作用。认为蚯蚓的单细胞凝胶电泳技术能敏感地农药对细胞ＤＮＡ的损伤，并可作为农药对土壤污
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| 关 键 词： | 农药 蚯蚓 单细胞凝胶电泳技术 DNA损伤 |
SINGLE CELL GEL ELECTROPHORESIS ASSAY IN THE EARTHWORM FOR MEASURING THE GENOTOXITY OF PESTICIDES TO DNA |
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| FENG Shao-long,LUO Yu,ZHONG Yuan,XIE chao-yun,KONG Zhi-ming. SINGLE CELL GEL ELECTROPHORESIS ASSAY IN THE EARTHWORM FOR MEASURING THE GENOTOXITY OF PESTICIDES TO DNA[J]. Journal of Nanjing University: Nat Sci Ed, 2000, 36(5): 649-652 |
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| Authors: | FENG Shao-long LUO Yu ZHONG Yuan XIE chao-yun KONG Zhi-ming |
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| Abstract: | In this paper,the DNA damages of earthworms that were caused by pesticides were measured with single cell gel assay,which is an advanced technique for measuring and analyzing DNA breakage in mammalian cell. Earthworms can pass on pollutions between terrigenous and soil organisms,so they can show the changes of soil environment.And it is an important indicator of the safety of pesticides to eco environment.The single cell gel assay of earthworm cell was employed to evaluate the effects of pesticides on eco environment.The results showed two new esticides can damage the DNA of earthworms.We found that the single cell gel assay by earthworm cell can sensitively detect DNA damages that were caused by pesticides.And it can be used as a sensitive indicator of the effects of pesticides on soil. |
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| Keywords: | pesticide earthworm single cell gel electrophoresis assay (SCGE) |
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