| Effect of Mn cluster on the formation of superoxide radicals in photoinhibition of photosystem Ⅱ |
| |
| 摘 要： | To further realize the action of superoxide radicals (O2− .) in photoinhibition of photosystem Ⅱ (PSⅡ), we employed 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trap, associated with EPR spectroscopy, to study the effect of illumination time on O2− . formation during high light photoinhibition in PSⅡ membranes and Mn-depleted PSⅡ membranes. Results indicated that the removal of Mn cluster from PS membranes has a strong influence on the dynamics of superoxide formation. The relative mechanism was also discussed. These novel findings may further promote the studies of the structure and function of PSⅡ and the mechanism of photoinhibition.
|
| 关 键 词： | Mn cluster photosystem Ⅱ photoinhibition superoxide radicals EPR. |
| 收稿时间： | 2001-06-28 |
Effect of Mn cluster on the formation of Superoxide radicals in photoinhibition of photosystem II |
| |
| Suping Zhang,Tiecheng Tu,Jun Weng,Jingxi Pan,Chunhe Xu,Side Yao. Effect of Mn cluster on the formation of Superoxide radicals in photoinhibition of photosystem II[J]. Chinese science bulletin, 2001, 46(22): 1877-1880. DOI: 10.1007/BF02901162 |
| |
| Authors: | Suping Zhang Tiecheng Tu Jun Weng Jingxi Pan Chunhe Xu Side Yao |
| |
| Affiliation: | (1) Laboratory of Photosynthesis, Institute of Plant Physiology, Chinese Academy of Sciences, 200032 Shanghai, China;(2) Laboratory of Radiation Chemistry, Institute of Nuclear Research, Chinese Academy of Sciences, 201800 Shanghai, China |
| |
| Abstract: | To further realize the action of Superoxide radicals (O 2 -. ) in photoinhibition of photosystem H (PS II), we employed 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a spin trap, associated with EPR spectroscopy, to study the effect of illumination time on O 2 -. formation during high light photoinhibition in PS II membranes and Mn-depleted PS II membranes. Results indicated that the removal of Mn cluster from PS II membranes has a strong influence on the dynamics of Superoxide formation. The relative mechanism was also discussed. These novel findings may further promote the studies of the structure and function of PS II and the mechanism of photoinhibition. |
| |
| Keywords: | Mn cluster photosystem II photoinhibition Superoxide radicals EPR |
| 本文献已被 SpringerLink 等数据库收录！ |
| 点击此处可从《科学通报(英文版)》浏览原始摘要信息 |
|
点击此处可从《科学通报(英文版)》下载全文 |
