原发性肝癌组织中MC与MV计数关系

目的 研究原发性肝癌PHC）组织中肥大细胞（MC）和微血管（MV）计数及其临床病理意义，探讨二者之间的相互关系.方法 应用ABC免疫组化法对47例PHC手术切除标本常规石蜡包埋切片分别检测MV和MC，并在高倍镜下计数.结果 47例PHC癌组织中MV、MC计数均显著高于癌旁组织（MV：67.30±13.68 vs.37.20±10.58,p<0.01;MC：15.46±4.54 vs.8.08±2.03,p<0.01）；术前AFU（α-岩藻糖）≤10　μg/mL的病例及伴转移病例癌组织MV计数显著高于AFU>10 μg/mL病例及无转移病例（AFU：70.41±13.03 vs.59.97±12.69，p<0.05；转移：73.50±13.77个/HP vs.64.10±12.68，p<0.05），MC和MV在PHC中的计数与PHC其他主要临床病理特征无明显关系；癌组织中MC计数与MV计数呈密切正相关（r=0.348，p<0.05）.结论 MC，MV计数与PHC的发生、进展关系密切，且MC具有显著的促血管生成作用.

Study on the Relationship Between the Counts of MC and MV in Tissues of Primary Hepatocarcinoma(PHC)

ObjectiveTo study on the counts of microvessel(MV) and mast cell(MC) in the tissues of primary hepatocarcinoma(PHC) and detect the relation to the clinicopathological features of PHC,and the relationship between the counts of MC and MV.MethodsThe stains of MV and MC were completed by the immunohistochemical method of avidin-biotin complex in routine paraffin-embedded sections from 47 cases with PHC.ResultsThe count means of MV and MC in the cancer tissues were significantly higher than that in the peri-cancerous tissues(MV,67.30±13.68/HP vs.37.20±10.58 /HP,p<0.01;MC,15.64±4.54/HP vs 8.08±2.03 /HP,p<0.01);the count of MV in the patients with AFU(pre-operation) ≤10　μg/mL  in serum(70.41±13.03) was significantly higher than that whose AFU >10 μg/mL(59.97±12.69)(p<0.05) and the count of MV in the patients with metastasis(73.50±13.77) was significantly higher than that of non-metastasis(64.10±12.68) (p<0.05).There were no correlation among the count of MV,MC and the other main clinicopathological features of PHC;the count of MC was significantly correlated with the count of MV（r=0.348，p<0.05）.ConclusionsThe count of MC and MV might be closely related to the carcinogenesis and the progress of PHC;MC might be closely related to enhance the tumor angiogenesis.