Co-transformation to tobacco of Cre/lox site-specific recombination system and its precise recombination |
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Authors: | Lei Li Songmei Liu Yuanlei Hu Zhongping Lin |
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Institution: | (1) National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, 100871 Beijing, China;(2) Present address: College of Biology, Beijing Forest University, 100083 Beijing, China |
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Abstract: | For the temporally and spatially regulated expression of the barnase gene in plant, two kinds of plasmids with cre gene and
its directly repeat recognition sitesiox from bacteriophage P1 were constructed and co-transformed into tobacco by agrobacterium mediated procedure. The transgenic
plants were conformed by PCR analysis. The blocking fragment between the twolox directly repeat sites was excised by Cre protein in the transgenic plant genome. Cloning and sequencing the DNA fragment
from the co-transformed plant DNA showed that the precise DNA excision occurred in transgenic tobacco genome directed by Cre/lox site-specific recombination. |
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Keywords: | Cre/lox site-specific recombination system tobacco co-transformation precise recombination |
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