| 荞麦花粉肽及其类似物的合成、结构和免疫活性研究 |
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| 引用本文： | 王霞,刘俊达,王舒,宋艳玲. 荞麦花粉肽及其类似物的合成、结构和免疫活性研究[J]. 北京大学学报(自然科学版), 1999, 35(2): 195-200 |
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| 作者姓名： | 王霞 刘俊达 王舒 宋艳玲 |
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| 作者单位： | 北京大学化学与分子工程学院，北京，100871；北京医院老年所，北京，100730 |
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| 基金项目： | 国家自然科学基金;29672002; |
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| 摘 要： | 用Merrifield固相法合成了荞麦花粉肽BPP-1及其类似物BPP-1-3，BPP-1-4和BPP-2。通过FT-IR和CD谱初步讨论了BPP-1的二级结构，结果表明其水溶液构象属于无规卷曲和 β-折叠，但在三氟乙醇中，CD谱在206nm和222nm附近呈双负峰形，分子形成了α-螺旋构象。研究了BPP-1，BPP-1-3和BPP-2对动物和人淋巴细胞的增殖应答功能和抑制释放sIL-2R的作用，结果显示它们具有较强的促免疫活性。
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| 关 键 词： | 荞麦花粉 多肽 CD谱 免疫活性 |
| 收稿时间： | 1998-02-09 |
| 修稿时间： | 1998-02-09 |
Studies of Buckwheat Pollen Peptides and its Analogues on Synthesis ,Structure and Immune Activity |
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| WANG Xia,LIU Junda,WANG Shu,SONG Yanling. Studies of Buckwheat Pollen Peptides and its Analogues on Synthesis ,Structure and Immune Activity[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1999, 35(2): 195-200 |
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| Authors: | WANG Xia LIU Junda WANG Shu SONG Yanling |
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| Affiliation: | Department of Chemistry, Peking University, Beijing, 100871; Department of Bio-message and Modulation, Beijing Hospital, Institute of Geriatrics, Beijing, 100730 |
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| Abstract: | Buckwheat pollen peptide (BPP-1) and its three analogues were synthesized using Merrifield solid phase method. The secondary structure of BPP-1 in solution was studied by FT-IR and CD spectra. It was considered that the conformation of the peptide was a random coil and β-sheet. In TFE, characteristic negative extrema near 222 and 206nm were observed in its CD spectra. It seems that the BPP-1 is in the form of a α-helix. The effect of these peptides on spleen lymphocytes from mice and on release of sIL-2R from human lymphocytes were studied. The results showed that these peptides exhibit certain immune activity. |
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| Keywords: | buckwheat pollen peptide CD spectra immune activity |
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