| 怀地黄离体培养再生植株及其生长调控 |
| |
| 引用本文： | 李明军,张嘉宝.怀地黄离体培养再生植株及其生长调控[J].河南师范大学学报(自然科学版),1996,24(4):60-63. |
| |
| 作者姓名： | 李明军 张嘉宝 |
| |
| 作者单位： | 河南师范大学生物系 |
| |
| 基金项目： | 河南省科委青年自然科学基金 |
| |
| 摘 要： | 本文对怀地黄愈伤组织的诱导、分化和再生植株的生长调控进行了研究．结果表明：（１）６－ＢＡ１ｍｇ／Ｌ（单位下同）＋ＮＡＡ０．１的组合有利于叶片愈伤组织的形成，２，４－Ｄ２＋ＮＡＡ２的组合有利于根茎愈伤组织的形成；（２）诱导愈伤组织产生再生植株的最佳组合为６－ＢＡ１＋ＮＡＡ０．０１；（３）ＰＰ３３３对再生植株的生长调控具有良好的效应，ＰＰ３３３１处理后，再生植株生长健壮，根系发达，叶色浓绿．
|
| 关 键 词： | 怀地黄 愈伤组织 再生植株 PP_（333） |
In Vitro Culture of Rehmannia glutinosa and Formation and Growth Regulation of Regenerated Plantlet |
| |
| Li Mingjun,Zhang Jiabao,Liu Ping.In Vitro Culture of Rehmannia glutinosa and Formation and Growth Regulation of Regenerated Plantlet[J].Journal of Henan Normal University(Natural Science),1996,24(4):60-63. |
| |
| Authors: | Li Mingjun Zhang Jiabao Liu Ping |
| |
| Abstract: | This paper deals with studying on the tissue culture of rehmannia glutinosa, includinginduction of callus and formation of regenerated plantlet. Results showed: (1) 6 - BA1mg/1+ NAA0. 1mg/L combinations were advantageous to leaf callus inclution and 2, 4 - D2mg/L+NAA 2mg/L combinations were due to root-stem callus induction in callus induction; (2) 6 -BA and NAA combination were advantageous to callus differentiated regenerated plantlet. The optimal of 6 - BA and NAA were 1 mg/L and 0. 01mg/L respectively, which induced a lot of plantlets; (3)The regenerated plantlets treated with 1mg/L PP333 grewrobustly and had their developed root systems and robust green leaves. |
| |
| Keywords: | rehmannia glutinosa callus regenerated plantlet PP_(333) |
| 本文献已被 CNKI 维普 等数据库收录！ |
