| 怀地黄离体培养再生植株及其生长调控 |
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| 引用本文： | 李明军 张嘉宝. 怀地黄离体培养再生植株及其生长调控[J]. 河南师范大学学报(自然科学版), 1996, 24(4): 60-63 |
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| 作者姓名： | 李明军 张嘉宝 |
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| 作者单位： | 河南师范大学生物系 |
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| 基金项目： | 河南省科委青年自然科学基金 |
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| 摘 要： | 本文对怀地黄愈伤组织的诱导、分化和再生植株的生长调控进行了研究．结果表明：（１）６－ＢＡ１ｍｇ／Ｌ（单位下同）＋ＮＡＡ０．１的组合有利于叶片愈伤组织的形成，２，４－Ｄ２＋ＮＡＡ２的组合有利于根茎愈伤组织的形成；（２）诱导愈伤组织产生再生植株的最佳组合为６－ＢＡ１＋ＮＡＡ０．０１；（３）ＰＰ３３３对再生植株的生长调控具有良好的效应，ＰＰ３３３１处理后，再生植株生长健壮，根系发达，叶色浓绿．
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| 关 键 词： | 怀地黄；愈伤组织；再生植株；PP_（333） |
In Vitro Culture of Rehmannia glutinosa and Formation and Growth Regulation of Regenerated Plantlet |
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| Li Mingjun,Zhang Jiabao,Liu Ping. In Vitro Culture of Rehmannia glutinosa and Formation and Growth Regulation of Regenerated Plantlet[J]. Journal of Henan Normal University(Natural Science), 1996, 24(4): 60-63 |
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| Authors: | Li Mingjun Zhang Jiabao Liu Ping |
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| Abstract: | This paper deals with studying on the tissue culture of rehmannia glutinosa, includinginduction of callus and formation of regenerated plantlet. Results showed: (1) 6 - BA1mg/1+ NAA0. 1mg/L combinations were advantageous to leaf callus inclution and 2, 4 - D2mg/L+NAA 2mg/L combinations were due to root-stem callus induction in callus induction; (2) 6 -BA and NAA combination were advantageous to callus differentiated regenerated plantlet. The optimal of 6 - BA and NAA were 1 mg/L and 0. 01mg/L respectively, which induced a lot of plantlets; (3)The regenerated plantlets treated with 1mg/L PP333 grewrobustly and had their developed root systems and robust green leaves. |
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| Keywords: | rehmannia glutinosa callus regenerated plantlet PP_(333) |
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