| Quantitative Estimates of Outcrossing Rates in a Natural Population of Caldesia grandis （Alismataceae） |
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| 引用本文： | Gituru Wahiti Robert. Quantitative Estimates of Outcrossing Rates in a Natural Population of Caldesia grandis （Alismataceae）[J]. 武汉大学学报:自然科学英文版, 2007, 12(2): 337-342. DOI: 10.1007/s11859-006-0004-6 |
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| 作者姓名： | Gituru Wahiti Robert |
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| 作者单位： | Botany Department |
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| 基金项目： | Supported by the State Key Basic Research and Development Plan （G2000046805） and the National Natural Science Foundation of China （30370098, 30570111）. The authors thank Wang Jingyuan for his help in fieldwork and Xia Jing and Dong Yuanhuo for their assistance in the laboratory. |
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| 摘 要： | Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA (RAPD) marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus estimates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates (t_m) and single-locus outcrossing rates (t_s) obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of t_m.
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| 关 键 词： | 泽泻科 泽苔草 天然种群 远交率 RAPD 定量估计 |
| 文章编号： | 1007-1202（2007）02-0337-06 |
| 收稿时间： | 2006-03-04 |
Quantitative estimates of outcrossing rates in a natural population of Caldesia grandis (Alismataceae) |
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| Chen Jinming,Gituru Wahiti Robert,Wang Qingfeng. Quantitative estimates of outcrossing rates in a natural population of Caldesia grandis (Alismataceae)[J]. Wuhan University Journal of Natural Sciences, 2007, 12(2): 337-342. DOI: 10.1007/s11859-006-0004-6 |
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| Authors: | Chen Jinming Gituru Wahiti Robert Wang Qingfeng |
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| Affiliation: | (1) College of Life Sciences, Wuhan University, Wuhan, 430072, Hubei, China;(2) Botany Department, Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya |
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| Abstract: | Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA (RAPD) marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ± 0.033 and the single-locus outcrossing rate was 0.795 ± 0.032. Multilocus estimates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was −0.142 ±0.000. The estimates of multilocus outcrossing rates (t m) and single-locus outcrossing rates (t s) obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of t m. Biography: CHEN Jinming (1975–), male, Ph.D., research direction: plant systematics and evolutionary biology. |
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| Keywords: | Alismataceae clonal plant Caldesia grandis mating system random amplified polymorphism DNA(RAPD) |
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