Construction
of cDNA library, nucleotide sequence and analysis of entire genome of classical swine
fever virus strain Shimen |
| |
Abstract: | According
to the previously published CSFV sequences, 18 pairs of primers have been designed and
synthesized, which cover the entire genome of CSFV strain Shimen. Each cDNA fragment has
been amplified by RT-PCR from the anticoagulant blood of strain Shimen infected pig. The
PCR products have been cloned respectively and sequenced. Results show that the cDNA
library of strain Shimen and its nucleotide sequence have been obtained. The genomic RNA
of strain Shimen is 12 298 nucleotides in length, containing a 5' and a 3' noncoding
region 373 and 231 nt long respectively. The center of genome is a single large open
reading frame of 11 697 nt which encodes a polyprotein of 3 898 amino acids. The entire
sequence of strain Shimen has also been compared with that of other CSFV strains. |
| |
Keywords: | |
|
|