Characterization of the flagellar biosynthesis regulatory geneflbD inAzospirillum brasilense |
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Authors: | Juan Wang Dalai Yan Jilun Li |
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Affiliation: | (1) National Laboratory for Agrobiotechnology, China Agricultural University, 100094 Beijing, China;(2) Department of Plant and Microbial Biology, University of California, 94720 Berkeley, CA, USA |
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Abstract: | A flagellar gene cluster fragment includingflbD ofAzospirillum brasilense was cloned and sequenced. TheflbD mutant strain was found to be nonmotile—losing both polar and lateral flagella (Fla−Laf−). Motility and flagella were regained by complementation with plasmid-borne multicopyflbD, but altered with larger swarming circle and fewer lateral flagella on the semisolid plate. This result indicated that FlbD plays an important role in the regulation of both polar and lateral flagellar biosynthesis inA. brasilense. |
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Keywords: | Azospirillum brasilense flbD gene flagella |
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