| 基于表面吸附固定法构制孔雀石绿免疫传感器的研究 |
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| 引用本文： | 周亚民,彭敏.基于表面吸附固定法构制孔雀石绿免疫传感器的研究[J].东莞理工学院学报,2012,19(5):64-69. |
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| 作者姓名： | 周亚民 彭敏 |
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| 作者单位： | 东莞理工学院化学与环境工程学院,广东东莞,523808 |
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| 基金项目： | 东莞市科技项目，广东省教育部产学研结合项目 |
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| 摘 要： | 提出一种基于石蜡电极表面吸附孔雀石绿抗体的电化学免疫传感器,通过竞争型免疫检测法测量水体中残留孔雀石绿。对免疫传感器构制参数——电极表面预处理、抗-MG溶液pH、抗-MG溶液浓度和抗-MG抗体吸附时间进行了研究;对传感器测量过程参数进行了优化,传感器对孔雀石绿检测的线性范围为1.5～780μg/L,检测孔雀石的回收率在93%～116%。
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| 关 键 词： | 孔雀石绿 免疫传感器 石蜡电极 |
Malachite Green Immunosensor Based on the Adsorptive Immobilization Technique |
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| ZHOU Ya.min PENG Min.Malachite Green Immunosensor Based on the Adsorptive Immobilization Technique[J].Journal of Dongguan Institute of Technology,2012,19(5):64-69. |
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| Authors: | ZHOU Yamin PENG Min |
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| Institution: | ZHOU Ya.min PENG Min (College of Chemistry and Environmental Engineering, Dongguan University of Technology, Dongguan 523808, China) |
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| Abstract: | This paper proposes amperometric immunosensor based on the adsorptive immobilization of Malachite Green anti- body on the solid paraffin electrode surface, measuring residues of Malachite Green in water bodies by competitive immuno - detec- ted method. Meanwhile, it studies Fabrication parameters of the immune sensor （ electrode surface pretreatment, pH of anti - MG o- solution, anti - MG solution concentration and anti - MG antibody adsorption time）, optimizing parameters of sensor in measuring course, and obtaining 1.5 -780 g/L of Malachite Green measuring range and 93% - 116% of recovery rate. |
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| Keywords: | tire industry cost control inventory just-in-time production |
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