利用扇贝边制造水解动物蛋白的研究

选用复合蛋白酶酶解扇贝边制备水解液，并优化酶解工艺条件；采用中空纤维超滤膜对水解液进行分离纯化，制造高质量的水解动物蛋白，并测试膜分离试验操作条件对分离效率的影响．研究结果表明，先用枯草杆菌蛋白酶酶解4h，然后用风味蛋白酶继续酶解，可以显著提高酶解液中多肽、短肽及游离氨基酸的含量；超过滤的操作压力、温度、酶解液的pH值及浓缩比等因素都将影响超过滤效率，在操作压力不高于0．10MPa，pH值为7．0～7．5，温度为43℃，每过滤10min后就对超过滤膜进行2min的反冲洗等操作条件下，可以大大提高超过滤的分离效率、减少膜的污染及延长膜的使用寿命；试验结果还表明，超过滤分离后水解液的感官指标明显优于未分离的水解原液。

Manufacture of Hydrolyzed Animal Protein from Scallop Skirt

After hydrolysis with mixed protease, the hydrolysates of scallop skirt was purified by hollow fi-ber ultrafiltration(UF). The processing parameters for the manufacture of liquid-HAP in good quality wereinvestigated. Results show that the content of polypeptides, peptides, and free amino acids in the liquid-HAPis largely increased by using flavorzyme after 4 h pre-hydrolysis with A. S. 1. 398 neutral subtilisin. And thatpermeation flux was affected by operating pressure, temperature, pH value of hydrolysates, and the ratio ofvolume to concentration (RVC). Optimal operative condition, which might prolong working time of the de-vice with high permeation flux, minimize pollution of membranes and raise separating efficiency, is pH 7.0～ 7.5 at 43 ℃ under 0.10 MPa and the membranes rinsed repeatedly for 2 minutes after every operating for10 minutes. These results suggest that sense index of fractionated hydrolysates is superior to that of the origi-nal hydrolysates.