Ca-ALC络合物和鱼精子DNA反应的电化学与光谱研究

在pH为6．0的六次甲基四胺缓冲溶液中，Ca—ALC(茜素)络合物与鱼精子DNA在室温下2min内结合生成了非电活性的超分子化合物，导致络合物的峰电流降低和峰电位的负移，峰电流值在一定范围内与DNA的浓度成线性关系，线性范围为0．1～3μg／mL，方法可以用于DNA的测定．和其它方法相比，该方法简便，快速，有较强的选择性和实用性．在加入DNA的条件下，电化学参数(电子转移系数α，标准速率常数Ks)显示了较大的改变．同时，结合荧光法和UV光谱法，推断反应的结合方式可能为嵌入结合．

Studies of Ca-ALC interacting with herring sperm DNA by electrochemical,fluorimetric and UV-spectrophotometric method

In the buffer solution of (CH2)6N4(pH=6.0), Ca-ALC(alizarin complexone) complex can react with herring sperm DNA(hsDNA) to form an electrochemically non-active supermolecular complex DNA-Ca-ALC within 2 min at room temperature, which results in the maximum decrease of the peak current of Ca-ALC and a negative shift of the peak potential. The Ip is proportional to hsDNA concentration from 0.1 to 3 μg·mL-1. So this method can be used for the determination of DNA. Compared with some other methods, the procedure is simple, rapid, selective and practical. In the presence of DNA, the electrochemical parameters(the electron transfer coefficient α, the standard rate constant Ks) show significant change. In addition, by the use of fluorimetric and UV-spectrophotometric methods, it is assumed that the binding mode is intercalation.