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| Corticosterone activates Erk1/2 mitogen-activated protein kinase in primary hippocampal cells through rapid nongenomic mechanism | |
| 作者姓名： | QI Aiqun QIU Jian XIAO Lin CHEN Yizhang |
| 作者单位： | Department of Physiology, Second Military Medical University, Shanghai 200433, China,Department of Physiology, Second Military Medical University, Shanghai 200433, China,Department of Neurobiology, Second Military Medical University, Shanghai 200433, China,Department of Neurobiology, Second Military Medical University, Shanghai 200433, China;Institute of Neuroscience, Second Military Medical University, Shanghai 200433, China |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
| 摘 要： | Nongenomic effects of glucocorticoids (GC) in various cell types have been well documented, but it still remains unknown whether the mechanism also works in hippocampus which is a crucial target of glucocorticoids in neural system during physiological and/or pathophysiological processes. We present here that corticosterone (B) could rapidly activate Erk1/2 mitogen-activated protein kinase (MAPK) in primarily cultured hippocampal cells within minutes, with a bell-shaped time dependent curve which peaked at 15min and then went down to normal level in 30 min. This activation was blocked by protein kinase C (PKC) inhibitor (Go6976), G protein inhibitor (GDPβs), and MEK(MAPK/extracellular signal-regulated kinase kinase) inhibitor(PD98059), but not by protein kinase A (PKA) inbibitor (H89), tyrosine kinase inhibitor (genistein), and glucocorticoid receptor ( GR ) antagonist (RU38486). Thus, the rapid activation of Erk1/2 MAPK in primary hippocampal cells induced by B was likely mediated by a G protein coupled receptor (GPCR) pathway with involvement of PKC, which belonged to the nongenomic rather than genomic mechanism of GC' s effects. |
| 关 键 词： | glucocorticoid MAPK rapid effects nongenomic mechanism |
Corticosterone activates Erk1/2 mitogen-activated protein kinase in primary hippocampal cells through rapid nongenomic mechanism |
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| QI Aiqun,QIU Jian,XIAO Lin,CHEN Yizhang.Corticosterone activates Erk1/2 mitogen-activated protein kinase in primary hippocampal cells through rapid nongenomic mechanism[J].Progress in Natural Science,2005,15(4):325-330. | |
| Authors: | QI Aiqun QIU Jian XIAO Lin CHEN YiZhang |
| Institution: | 1. Department of Physiology, Second Military Medical University, Shanghai 200433, China 2. Department of Neurobiology, Second Military Medical University, Shanghai 200433, China 3. Department of Neurobiology, Second Military Medical University, Shanghai 200433, China;Institute of Neuroscience, Second Military Medical University, Shanghai 200433, China |
| Abstract: | Nongenomic effects of glucocorticoids (GC) in various cell types have been well documented, but it still remains unknown whether the mechanism also works in hippocampus which is a crucial target of glucocorticoids in neural system during physiological and/or pathophysiological processes. We present here that corticosterone (B) could rapidly activate Erk1/2 mitogen-activated protein kinase (MAPK) in primarily cultured hippocampal cells within minutes, with a bell-shaped time dependent curve which peaked at 15min and then went down to normal level in 30 min. This activation was blocked by protein kinase C (PKC) inhibitor (Go6976), G protein inhibitor (GDPβs), and MEK(MAPK/extracellular signal-regulated kinase kinase) inhibitor(PD98059), but not by protein kinase A (PKA) inbibitor (H89), tyrosine kinase inhibitor (genistein), and glucocorticoid receptor ( GR ) antagonist (RU38486). Thus, the rapid activation of Erk1/2 MAPK in primary hippocampal cells induced by B was likely mediated by a G protein coupled receptor (GPCR) pathway with involvement of PKC, which belonged to the nongenomic rather than genomic mechanism of GC' s effects. |
| Keywords: | glucocorticoid MAPK rapid effects nongenomic mechanism |
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