| 好好芭叶片RNA提取方法和cDNA干旱消减文库的构建 |
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| 引用本文： | 耿红卫,王纯,周宜君,张根发.好好芭叶片RNA提取方法和cDNA干旱消减文库的构建[J].北京师范大学学报(自然科学版),2007,43(1):79-82. |
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| 作者姓名： | 耿红卫 王纯 周宜君 张根发 |
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| 作者单位： | 北京师范大学生命科学学院,100875,北京;中央民族大学生命与环境科学学院,100081,北京 |
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| 基金项目： | 国家自然科学基金，北京市自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | 介绍了从好好芭成熟叶片中提取总RNA的方法;用含有SDS的裂解液裂解细胞,用DNase除去可能出现的DNA干扰,再经植物提取试剂盒纯化后,获得了高质量的完整RNA,可用于基因编码序列的克隆和基因表达的mRNA分析等精细研究,并进一步构建了好好芭干旱消减文库.
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| 关 键 词： | 好好芭 成熟叶片 总RNA提取 抑制消减杂交 |
| 修稿时间： | 2006-10-24 |
AN IMPROVED METHOD FOR EXTRACTION OF TOTAL RNA FROM MATURE LEAVES OF JOJOBA AND SUBTRACTION cDNA LIBRARY CONSTRUCTION |
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| Geng Hongwei,Wang Chun,Zhou Yijun,Zhang Genfa.AN IMPROVED METHOD FOR EXTRACTION OF TOTAL RNA FROM MATURE LEAVES OF JOJOBA AND SUBTRACTION cDNA LIBRARY CONSTRUCTION[J].Journal of Beijing Normal University(Natural Science),2007,43(1):79-82. |
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| Authors: | Geng Hongwei Wang Chun Zhou Yijun Zhang Genfa |
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| Institution: | 1College of Life Science, Beijing Normal University, 100875, Beiiing, China; 2College of Life and Environmental Sciences, Central University for Nationalities, 100081, Beijing, China |
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| Abstract: | A method to extract total RNA from jojoba mature leaf is presented.SDS-containing lysis buffer is proved appropriate for leaf cell disruption. DNase is used to remove genome DNA contamination.High quality total RNA can be obtained after purification by plant RNA preparation kit,which can be used for cloning of cDNA coding sequence and study of gene expression at mRNA level.Then a drought-regulated suppression subtractive hybridization library is constructed from jojoba leaves treated by drought. |
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| Keywords: | jojoba mature leaf total RNA extraction SSH |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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