| 波氏假丝酵母启动子的克隆及新霉素基因的表达 |
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| 引用本文： | 徐柏年,吴江雪,李文清,罗进贤.波氏假丝酵母启动子的克隆及新霉素基因的表达[J].中山大学学报(自然科学版),2001,40(1):65-68,76. |
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| 作者姓名： | 徐柏年 吴江雪 李文清 罗进贤 |
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| 作者单位： | 中山大学生命科学学院 |
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| 基金项目： | 广东省自然科学基金项目资助项目(970175) |
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| 摘 要： | 报道了波氏假丝酵母（Candida boidinii)启动子的克隆，将克隆的启动子与Neo^ 基因重组构建了含Neo^r基因的表达载体YepNP181，转化波氏假丝酵母，实现了Neo^r基因在波氏假丝酵母中的表达，使波氏假丝酵母转化子能在含G418的抗性培养基上生长从而建立了Neo^r基因－G418选择系统，为外源基因在波氏假丝酵母中表达创造了条件。
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| 关 键 词： | 启动子克隆 新霉素抗性基因 波氏假丝酵母 基因表达 工业发酵菌种 G418选择系统 转化子 |
| 文章编号： | 0529-6579(2001)01-0065-04 |
Cloning of Promoter from
Candida boidinii and the Expression of Neomycin Resistant Gene |
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| XU Bai-nian,WU Jiang-Xue,LI Wen-qing,LUO Jin-xian.Cloning of Promoter from
Candida boidinii and the Expression of Neomycin Resistant Gene[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2001,40(1):65-68,76. |
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| Authors: | XU Bai-nian WU Jiang-Xue LI Wen-qing LUO Jin-xian |
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| Abstract: | Candida boidinii is one of the methylotrophic yeast with good development and application perspective but very little study has been done as a gene expression system. The C. boidinii promoter was cloned and the expression vector YEpNP181 was constructed by recombining the cloned promoter and Neor gene onto an E. coli-yeast shuttle vector. YEpNP181 was then transformed into C. boidinii and Neor gene on YEpNP181 expressed. The transformants grow on YPD plate containing 300 μg/mL G418 and Neor-G418 selection system was thus esta blished, which facilitates the foreign gene expression in C. boidinii. |
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| Keywords: | promoter cloning Candida boidinii Neor gene expression |
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