| Application of microfluidic chip to realize controllable pH gradient |
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| 作者姓名： | LUOChunxiong SUNManhui XULuping ZHANGZhiling CCrozatier CHENYong JIHang |
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| 作者单位： | [1]CenterforMicrofluidicandNanotechnology,SchoolofPhysics,PekingUniversity,Beijing100871,China [2]ChemistryDepartmentofWuhanUniversity;Wuhan430072,China [3]EcoleNormaleSuperieure,24rueLhomond,75231Paris,France [4]CenterforMicrofluidicandNanotechnology,SchoolofPhysics,PekingUniversity,Beijing100871,China／EcoleNormaleSuperieure,24rueLhomond,75231Paris,France |
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| 摘 要： | A kind of PH gradient microfluidic chips through soft-lithography microfabrication for isoelectric focusing (IEF) and high performance liquid chromatography (HPLC) is introduced here. These pH gradient chips have the advantages such as easy fabrication, controllable pH range and precision, isoelectric focusing and separation at the same time, low voltage for isoelectric focusing, and time stable pH gradient. This method has potential application to sample preparation, separation and analysis of microfluidic chips.
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| 关 键 词： | 微流体 控制方法 PH梯度 平版印刷术 层流 石印术 电场 阴极 电化学方法 |
| 收稿时间： | 22 July 2004 |
Application of microfluidic chip to realize controllable pH gradient |
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| LUOChunxiong SUNManhui XULuping ZHANGZhiling CCrozatier CHENYong JIHang.Application of microfluidic chip to realize controllable pH gradient[J].Chinese Science Bulletin,2005,50(4):365-367. |
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| Authors: | Chunxiong Luo Manhui Sun Luping Xu Zhiling Zhang C Crozatier Yong Chen Hang Ji |
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| Institution: | (1) Center for Microfluidic and Nanotechnology, School of Physics, Peking University, 100871 Beijing, China;(2) Chemistry Department of Wuhan University, 430072 Wuhan, China;(3) Ecole Normale Superieure, 24 rue Lhomond, 75231 Paris, France |
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| Abstract: | A kind of PH gradient microfluidic chips through soft-lithography microfabrication for isoelectric focusing (IEF) and high performance liquid chromatography (HPLC) is introduced here. These pH gradient chips have the advan- tages such as easy fabrication, controllable pH range and precision, isoelectric focusing and separation at the same time, low voltage for isoelectric focusing, and time stable pH gradient. This method has potential application to sample preparation, separation and analysis of microfluidic chips. |
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| Keywords: | microfluidic pH gradient soft-lithography laminar flows lab on a chip |
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