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Efficient selection of homozygous lines of hybrid rice restorer with the transgeneXa21 using test cross and PCR
Authors:Bojun Ma  Wenming Wang  Junhuang Zou  Guoqing Liu  Jin Fang  Lihuang Zhu  Wenxue Zhai
Institution:(1)Institute of Genetics, Chinese Academy of Sciences ,Beijing 100101 ,China;(2)Zhejiang Normal University ,Jinhua 321004 ,China;(3)Yangzhou University ,Yangzhou 225009 ,China
Abstract:The homozygous restorer lines with a single copy of the transgene Xa21 have been obtained from the progenies of transgenic Minghui63 and Yanhui559 plants through PCR marker-assisted selection and test cross. These homozygous transgenic restorer lines can be used to breed hybrid rice with high resistance to bacterial leaf blight.
Keywords:test cross  PCR  marker-assisted selection  rice restorer with the transgene Xa21  homozygous
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