一种基于信息论的蛋白质数据库搜索鉴定算法

为了有效地利用蛋白质串联质谱数据,提高蛋白质鉴定的准确性,提出了一种基于信息论的蛋白质数据库搜索鉴定算法——ITPIA(information theory based protein identification algorithm)算法.针对多肽串联质谱质量低、噪音多等问题,ITPIA算法利用了信息论中的熵理论提出了一种有效的实验串联质谱和多肽的理论质谱的匹配打分算法.该算法更大程度上从多肽串联质谱中获得蛋白质的结构信息.实验结果表明,ITPIA算法有效地提高了蛋白质鉴定的准确性.

A Database Search Algorithm Based on Information Theory for Protein Identification

The ITPIA(information theory based protein identification algorithm) based on information theory is proposed to search protein database by use of polypeptide tandem mass spectra for more exact protein identification.To solve efficiently the problem of low-quality and noisy spectra,the entropy theory is introduced in the ITPIA with a scoring system built to measure the similarity between the experimental spectrum and the theoretical spectrum of polypeptide in the database. The algorithm thus acquires more in...