| Neurobasal~(TM)-A和DMEM/F12培养液对大鼠胎儿神经干细胞生长的影响 |
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| 引用本文： | 李雪玲,John R.Morrison,扎拉嘎胡,扈廷茂.Neurobasal~(TM)-A和DMEM/F12培养液对大鼠胎儿神经干细胞生长的影响[J].内蒙古大学学报(自然科学版),2001(6). |
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| 作者姓名： | 李雪玲 John R.Morrison 扎拉嘎胡 扈廷茂 |
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| 作者单位： | 内蒙古大学生命科学学院生物系，Monash Institute of Reproduction and Development，内蒙古大学生命科学学院生物系，内蒙古大学生命科学学院生物系 内蒙古呼和浩特010021，Clayton3168，Australia，内蒙古呼和浩特010021，内蒙古呼和浩特010021 |
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| 摘 要： | 用带有 3 H]的胸苷对分裂细胞进行标记 ,通过细胞计数仪测定细胞数量的方法 ,对比了大鼠胎儿神经干细胞在添加相同成分的 Neurobasal TM-A和 DMEM/F1 2培养液中的生长情况 .结果表明 Neurobasal TM-A在有 EGF和 b FGF存在的情况下更适合于神经干细胞的生长和存活
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| 关 键 词： | NeurobasalTM-A DMEM/F12 大鼠胎儿神经干细胞(rFNSC) |
Effects of Neurobasal~(TM)-A and DMEM/F12 Media on the Growth of Rat Fetal Neural Stem Cells |
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| LI Xue ling ,John R.Morrison ,Zhalagahu ,HU Ting mao.Effects of Neurobasal~(TM)-A and DMEM/F12 Media on the Growth of Rat Fetal Neural Stem Cells[J].Acta Scientiarum Naturalium Universitatis Neimongol,2001(6). |
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| Authors: | LI Xue ling John RMorrison Zhalagahu HU Ting mao |
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| Institution: | LI Xue ling 1,John R.Morrison 2,Zhalagahu 1,HU Ting mao 1, |
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| Abstract: | Rat fetal neural stem cells derived from E14.5~16.5 rat fetal brain, and cultured in Neurobasal TM A and DMEM/F12 mdia containing 10ng/ml EGF and 10ng/ml bFGF respectively.Data from 3 Thymidine incorporation shows that rat neural stem cells grow better and faster in Neurobasal TM A medium, and suggest Neurobasal TM A is another suitable medium for the neural stem cell culture. |
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| Keywords: | Neurobasal TM A medium DMEM/F12 medium rat neural stem cell (rFNSC) |
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