| 青枯菌SRAP—PCR体系的建立与优化 |
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| 引用本文： | 智艳平,武喜艳,安瑶瑶,郜刚. 青枯菌SRAP—PCR体系的建立与优化[J]. 山西师范大学学报：自然科学版, 2013, 0(4): 69-72 |
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| 作者姓名： | 智艳平 武喜艳 安瑶瑶 郜刚 |
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| 作者单位： | 山西师范大学生命科学学院,山西临汾041004 |
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| 基金项目： | 国家自然基金项目资助（31271774）. |
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| 摘 要： | 本研究以马铃薯青枯菌19046为试验材料，应用单因素试验法优化SRAP—PCR反应体系，得出重复性好、带型清晰、稳定性强的最佳反应体系：总体积为10μL，50．g／μL模板DNA0．6μ、10μmol／L引物0．8μL、TapDNA聚合酶1．0U、10mol／L、dNTPs1．0μL．该反应体系可用于青枯菌遗传多样性、基因定位与克隆等的研究．
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| 关 键 词： | 青枯菌 SRAP 体系优化 |
Establishment and Optimization of SRAP Analysis System of Ralstonia solanacearum Genome |
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| ZHI Yan-ping,WU Xi-yan,AN Yao-yao,GAO Gang. Establishment and Optimization of SRAP Analysis System of Ralstonia solanacearum Genome[J]. Journal of Shanxi Teachers University, 2013, 0(4): 69-72 |
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| Authors: | ZHI Yan-ping WU Xi-yan AN Yao-yao GAO Gang |
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| Affiliation: | ＊ (School of Life Science, Shanxi Normal University, Lirifen 041004, Shanxi, China) |
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| Abstract: | Some factors affecting PCR amplification of SRAP（Sequence-related amplified polymorphism） were studied using Ralstonia solanacearum P046. A reliable, effective,reproductive and legible repeats optimized technical system was developed. In the 10 μL PCR reaction nfixture,30 ng of genomic DNA, 1.0 U of Taq polymerase,0.8 μL 10 umol/L primers,and 1.0 μL dNTPs were used. This reaction system can be used to study genetic diversify,gene tagging cloning and genetics mapping for SRAP of differ- cnt Ralstonia solanacearum. |
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| Keywords: | Ralstonia solanacearum SRAP optimization |
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