固定化乙酰胆碱脂酶的研究

以戊二醛为交联剂，将乙酰胆碱脂酶交联固定到硅胶载体上，研究了影响固定化酶的主要因素．通过酶活力的测定，确定了最佳戊二醛浓度、pH值、固定化时间、酶用量、固定化酶的最适作用温度、pH值、酶的稳定性及其回收率．结果表明：戊二醛浓度为0．5％，pH值为8．0，固定3．0h，酶与载体比例为30mg／g可制备良好的功能化载体．固定化乙酰胆碱脂酶最适作用温度和pH范围分别为40℃和6．0～9．0．重复使用10次固定化酶，回收率约为70％．

Study on Immobilization of Acetylcholinesterase

Acetyleholinesterase was immobilized on the silica gel using glutaraldehyde as cross linker. The concentration of glutaraldehyde, pH, time and amounts of enzyme have been optimized by means of enzymeactiv- ity. The optimum reaction temperature, pH, stability and the activity yield of the immobilized acetylcholinester- ase have been studied. The results showed that the optimum concentration of glutaraldehyde and pH value were 0.5% and 8.0 respectively, the coupling capacity of acetylcholinesterase was 30mg/g silica gel. And the opti- mal temperature and the range of pH were 40℃ and 6.0 -9.0. The activity yield of the immobilized acetylcho- linesterase was about 70% after 10 cycles reactions.