| 白屈菜碱对SGC-7901细胞Cdk1、cyclinB1表达影响 |
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| 引用本文： | 季宇彬,孟凡影,曲中原,邹翔.白屈菜碱对SGC-7901细胞Cdk1、cyclinB1表达影响[J].哈尔滨商业大学学报(自然科学版),2012(1):1-3. |
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| 作者姓名： | 季宇彬 孟凡影 曲中原 邹翔 |
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| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究中心;国家教育部抗肿瘤天然药物工程研究中心 |
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| 基金项目： | 国家自然科学基金项目(81102858);教育部博士点基金项目(20102332120003);黑龙江省自然科学基金资助项目(D200817);黑龙江省骨干教师项目(1154G35) |
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| 摘 要： | 研究白屈菜碱对人胃癌SGC-7901细胞Cdk1、p-Cdk1(Thr14)、cyclinB1蛋白表达,探讨白屈菜碱诱导人胃癌SGC-7901细胞G2/M期阻滞的机制.采用Western blotting法测定白屈菜碱对SGC-7901细胞Cdk1、p-Cdk1(Thr14)、cyclinB1蛋白表达的影响.不同质量浓度的白屈菜碱可显著下调人胃癌SGC-7901细胞内Cdk1与cyclinB1蛋白表达水平,同时显著上调p-Cdk1(Thr14)蛋白的表达水平,并呈一定的剂量依赖性.白屈菜碱可下调SGC-7901细胞内Cdk1和cyclinB1蛋白的表达,上调p-Cdk1(Thr14)蛋白的表达,这可能是白屈菜碱诱导SGC-7901细胞G2/M期阻滞的主要机制之一.
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| 关 键 词： | 白屈菜碱 Cdk1 p-Cdk1(Thr14) cyclinB1 人胃癌SGC-7901细胞 |
Effect of chelidonium on expression of Cdk1,cyclinB1 protein in SGC-7901 cells |
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| JI Yu-bin,MENG Fan-ying,QU Zhong-yuan,ZOU Xiang.Effect of chelidonium on expression of Cdk1,cyclinB1 protein in SGC-7901 cells[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2012(1):1-3. |
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| Authors: | JI Yu-bin MENG Fan-ying QU Zhong-yuan ZOU Xiang |
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| Institution: | 1,2(1.Life Sciences and Environmental Sciences Research Center,Harbin University of Commerce,Harbin 150076,China;2.Key Research Laboratory of Cancer Prophylaxis and Anticancer Drugs,Harbin 150076,China) |
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| Abstract: | To study the effect of Chelidonium on Cdk1,p-Cdk1(Thr14),cyclinB1 protein expression in SGC-7901 Cells,and to explore the mechanism of Chelidonium inducing G2/M phase arrest in SGC-7901 Cells.Western Blotting was used to study the effects of Chelidonium on the Cdk1,p-Cdk1(Thr14) and cyclinB1 protein expressions in SGC-7901 cells.Chelidonium could significantly decrease the expression of Cdk1 and cyclinB1 protein.Meanwhile,Chelidonium could remarkably increase the expression of p-Cdk1(Thr14) protein in a dose dependent manner.Chelidonium can down-regulate the expression of Cdk1,cyclinB1 and up-regulate p-Cdk1(Thr14) protein expression in SGC-7901 cells,which may be one of the role mechanism of G2/M phase arrest induced by Chelidonium in SGC-7901 Cells. |
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| Keywords: | chelidonium Cdk1 p-Cdk1(Thr14) cyclinB1 human gastric carcinoma SGC-7901 cells |
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