全氟辛酸在小鼠肝脂质过氧化损伤中的作用

确定全氟辛酸是否通过脂质过氧化造成小鼠肝脏的损伤.将小鼠随机分为4组,分别为空白组、1/16LD50、1/8LD50和1/4LD50组,染毒14 d后采用HE染色法观察肝脏病理变化,试剂盒法测定肝组织匀浆中超氧化物歧化酶(SOD);还原型谷胱甘肽(GSH);丙二醛(MDA)、血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)含量变化.HE染色观察到全氟辛酸染毒组肝组织损伤明显;试剂盒结果显示,与空白组相比,全氟辛酸组小鼠组织匀浆中SOD活性显著降低,MDA含量显著升高,GSH活性显著降低,均有统计学意义.全氟辛酸组小鼠血清中ALT和AST活性明显高于空白组(P<0.01),低剂量组小鼠血清ALP活性与空白组相比显著升高(P<0.05),高、中剂量组与空白组相比ALP活性有升高趋势,但是没有统计学意义.全氟辛酸通过脂质过氧化造成小鼠肝损伤.

Effects of perfluorooctanoic acid on lipid peroxidation injury in liver of mice

To investigate the effects of PFOA on lipid peroxidation injury in liver of mice.Mice were divided into 4 groups randomly,the control group,1/16LD50,1/8LD50,1/4LD50.After exposure for 14 d,the liver pathological changes were observed by HE staining,superoxide dismutase(SOD);reduced glutathione(GSH);malondialdehyde(MDA),serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) were measured by the method of reagent’s directions.The liver pathological changes were obvious.Activity of SOD and GSH in the model group were lower remarkably than those in the control group(P<0.01).Activity of MDA in the model group were higher remarkably than those in the control group(P<0.01).Activity of ALP in low dose group were higher remarkably than those in the control group in serum of mice(P<0.05),Activity of ALP in high and medium dose group were higher than those in the control group,but no statistical significance.PFOA induced liver injury by lipid peroxidation.