| HPLC测定益肝颗粒中五味子甲素的质量浓度 |
| |
| 引用本文： | 杨大宇,李洪阳,邹海曼,王磊,刘懿,李彦冰.HPLC测定益肝颗粒中五味子甲素的质量浓度[J].哈尔滨商业大学学报(自然科学版),2012(3):274-276. |
| |
| 作者姓名： | 杨大宇 李洪阳 邹海曼 王磊 刘懿 李彦冰 |
| |
| 作者单位： | 黑龙江中医药大学药学院 |
| |
| 摘 要： | 建立测定益肝颗粒中五味子甲素质量浓度的方法.室温条件下超声提取五味子甲素,HPLC测定其质量浓度,SinoChrom ODS-BP色谱柱,以甲醇-水(87∶13)为流动相;检测波长为254 nm;柱温25℃;流速为1.0 mL/min;进样量10μL.五味子甲素进样量在0.2～1.0μg范围内与峰面积线性关系良好;结果平均加样回收率为98.82%,(n=3),RSD为1.19%.该方法灵敏、准确、重复性好,可用于益肝颗粒的质量控制.
|
| 关 键 词： | 益肝颗粒 五味子甲素 高效液相色谱法 质量浓度测定 |
HPLC determination of deoxyschizandrin content in Yigan partides |
| |
| YANG Da-yu,LI Hong-yang,ZOU Hai-man,WANG Lei,LIU Yi,LI Yan-bing.HPLC determination of deoxyschizandrin content in Yigan partides[J].Journal of Harbin University of Commerce :Natural Sciences Edition,2012(3):274-276. |
| |
| Authors: | YANG Da-yu LI Hong-yang ZOU Hai-man WANG Lei LIU Yi LI Yan-bing |
| |
| Institution: | (School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China) |
| |
| Abstract: | A method was determined to quantitative analyze the schisandrin A in the benefit liver particles.SinoChrom ODS-BP as chromatographic column,the mixture of methanol and water in a molar ratio of 87∶ 13 as the mobile phase,at the bond of 254 nm,on 25 ℃,in a flow rate of 1.0 mL/min and injection volume 10 μL,HPLC was carried out to determine the amount of schisandrin A that was obtained by ultrasonic method at room temperature.The result showed that there was a good linear relationship between the injection volume and area of bond,when the injection volume was in the range from 0.2 μg to 1.0 μg.The average results of injection volume recovery was 98.82%,(n=3) and the RSD was 1.19%.The method was sensitive and accurate,showed a good repeatability,and thus it could be used for controlling the quality of the benefit liver particles. |
| |
| Keywords: | Yigan partcles schisandrin A HPLC quantitative analyses |
| 本文献已被 CNKI 等数据库收录！ |
|
