| PCR用于孕牛外周血中胎儿SRY序列检测和奶牛早期胚胎性别鉴定 |
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| 引用本文： | 杨建民,王丽山,刘连瑞,蒋耀青. PCR用于孕牛外周血中胎儿SRY序列检测和奶牛早期胚胎性别鉴定[J]. 首都师范大学学报(自然科学版), 1995, 0(2) |
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| 作者姓名： | 杨建民 王丽山 刘连瑞 蒋耀青 |
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| 作者单位： | 中国科学院遗传研究所 |
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| 摘 要： | 根据文献报道的人，鼠，兔ＳＲＹ同源序列设计引物，从牛基因组中扩增出一段牛特异的ＳＲＹ序列，对该序列进行克隆测序后设计出一对雄牛特异的ＰＣＲ引物，利用此引物对奶牛早期胚胎细胞和妊娠晚期母牛外周血ＤＮＡ样品进行ＰＣＲ扩增，经分娩牛犊性别验证，本引物能够准确鉴别孕牛外周血中雄性胎儿的ＳＲＹ序列，并且对早期胚胎性别签定的准确率为８０％．
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| 关 键 词： | Sry序列，PCR，性别，产前诊断 |
Detection of Bovine Fetal Y-Specific Sry from Preimplanted Embryo Cells and Maternal Blood Using Polymerase Chain Reaction |
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| Yang Jianmin, Wang Lishan, Lu Lianrui, Jiang Yaoqing. Detection of Bovine Fetal Y-Specific Sry from Preimplanted Embryo Cells and Maternal Blood Using Polymerase Chain Reaction[J]. Journal of Capital Normal University(Natural Science Edition), 1995, 0(2) |
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| Authors: | Yang Jianmin Wang Lishan Lu Lianrui Jiang Yaoqing |
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| Abstract: | Based on the human, rat and rabbit SRY homologous sequence, a pair of primers was designed to amplify cattle Sry fragment with PCR. With our new male-specific cattle Sry sequence, a pair of primers specific to cattle Sry was developed to sexing preimplanted embryo cells and to detect bovine fetal Sry sequence from maternal peripheral blood. The results showed that bovine Y-specific Sry sequence can be detected directly from preimplanted embryo cells for sex manipulation and from maternal blood for non-invasive identification of fetal sex. |
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| Keywords: | srysequence PCR sex determination prenatal diagnosis. |
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