Effects of prolactin and growth hormone on DNA synthesis of rat mammary carcinomas in vitro |
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Authors: | Gloria Calaf de Iturri C W Welsch |
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Institution: | (1) Department of Anatomy, Michigan State University, 48824 East Lansing, Michigan, USA;(2) Present address: Department of Biology, Faculty of Science, University of Chile, Santiago, Chile |
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Abstract: | Summary Explants derived from mammary carcinomas of DMBA-treated female Sprague-Dawley rats were cultured for 5 days in Medium 199 containing insulin and corticosterone. The addition of ovine prolactin to the culture media resulted in a consistent significant increase in H3-thymidine incorporation into DNA. DNA synthesis of explants treated with either ovine or human growth hormone was intermediary to prolactin-treated cultures and control cultures. A combination of prolactin and human growth hormone often increased DNA synthesis above either hormone alone, suggesting a possible growth synergism between these peptides.Supported by NIH research grant No. CA-13777 and American Cancer Society research grant No. ET-59.NIH Research Career Development Awardee No. CA-35027. |
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