| 基于宏基因组方法对西藏传统发酵牦牛奶中微生物多样性的研究 |
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| 引用本文： | 孙志宏,刘文俊,张和平.基于宏基因组方法对西藏传统发酵牦牛奶中微生物多样性的研究[J].北京工商大学学报(自然科学版),2012,30(4):19-24. |
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| 作者姓名： | 孙志宏 刘文俊 张和平 |
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| 作者单位： | 内蒙古农业大学乳品生物技术与工程教育部重点实验室,内蒙古呼和浩特,010018 |
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| 基金项目： | 国家杰出青年基金资助项目(31025019);教育部创新团队发展计划资助项目(IRT0967) |
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| 摘 要： | 采用454高通量测序技术从宏基因组角度对西藏地区自然发酵牦牛奶中微生物多样性进行分析,以细菌16S rRNA的V3区和真菌的ITS区序列为扩增、测序靶点,分别获得3 051和3 396条高质量序列.研究发现,西藏自然发酵牦牛奶样品中真菌的丰度要大于细菌.在门的水平上,细菌和真菌分别由硬壁菌门(Firmicutes)和子囊菌门(Ascomycota)组成,而在属的水平上细菌和真菌的优势菌分别为乳杆菌属(Lactobacillus)和耐碱酵母属(Galactomyces).
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| 关 键 词： | 自然发酵牦牛奶 微生物多样性 测序 |
Study on Microbial Diversity in Naturally Fermented
Yak Milk of Tibet Based on Metagenomics |
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| SUN Zhi-hong,LIU Wen-jun and ZHANG He-ping.Study on Microbial Diversity in Naturally Fermented
Yak Milk of Tibet Based on Metagenomics[J].Journal of Beijing Technology and Business University:Natural Science Edition,2012,30(4):19-24. |
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| Authors: | SUN Zhi-hong LIU Wen-jun and ZHANG He-ping |
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| Institution: | (Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education, Inner Mongolia Agriculture University,Huhhot 010018,China) |
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| Abstract: | In this study,the microbial diversity of metagenomics in naturally fermented yak milk(kurut) were investigated using barcoded pyrosequencing.A total of 3 051 unique V3 variable regions of the 16S rRNA gene of bacterium and 3 396 unique ITS variable regions of fungi were analyzed from two kurut samples.The results indicated that the abundance of fungi was higher than bacteria in the kurut samples.The Firmicutes and Ascomycota were the most abundant bacteria and fungi phyla in kurut samples.At genus levels,the bacteria of kurut were largely composed of the Lactobacillus while Galactomyces was the dominant genus within the fungus of kurut. |
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| Keywords: | naturally fermented yak milk microbial diversity 454 pyrosequencing |
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