完全毁损型偏侧帕金森病大鼠模型的行为及影像特征

目的 研究完全毁损型偏侧帕金森病大鼠模型的行为和影像学改变。方法 建立完全毁损型偏侧帕金森病大鼠模型，观察模型大鼠在自然状态下和阿朴吗啡诱导中出现的异常行为学改变， Micro-PET模型大鼠神经系统功能改变。结果 帕金森病大鼠模型在行为学上表现了在自发状态下的患肢运动感觉能力降低；在阿朴吗啡诱导下出现对侧旋转，及异常不自主运动；影像学上表现为损毁侧纹状体内多巴胺转运蛋白缺失及毁损侧纹状体、皮层及丘脑的功能活性降低。结论 完全毁损型偏侧帕金森病大鼠模型表现为单侧肢体的运动感觉功能障碍和损毁侧纹状体内多巴胺能神经末梢的缺失及相应区域的脑功能降低,类似于人类严重的帕金森末期阶段强直运动功能障碍的表现。

Characterization of Behavioral and Image of Completely Unilaterally Lesioned Parkinson Rat Model

Objective The characterization of behavioral and image of completely unilaterally 6-hydoxydopamine-lesioned Parkinson rat model were investigated. Methods we gave two 6-hydoxydopamine injection into right medial forebrain bundle (MFB) to setup completely unilaterally Parkinson rat model and observered their abnormal behavioral under both nature and apomorphine used situation. we used the 11C-?-CFT and 18FDG Micro-PET to image change of the ability of the region of the rat brain . Results compared with the inactive side, the initiation of stepping movements by the left(contra-lateral)paw are greatly reduced, the initial time was prolonged and the rate of using left paw reduced when the rat in the nature situation; under the apomorphine inducing, the rats show the contraversive rotation and abnormal involuntary movement, such as limb dyskinesia ,axial dystonia and masticatory dyskinesia. The 11C-?-CFT image showed the depletion of presynaptic maker DAT in the lesion side of the striatum and the FDG image showed the reduced ability in the lesion side. Conclusion completely unilaterally Parkinson rat model showed extensive neuropathology and profound loss of sensorimotor, analogous to severely rigid-akinetic terminal stage of human disease.