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抗氧化剂对单线态氧(~1O_2)清除能力的一种生物评价方法
作者单位:;1.云南民族大学民族药资源化学国家民委-教育部重点实验室;2.云南民族大学云南省高校民族地区资源清洁转化重点实验室
摘    要:用微生物纸片法对抗氧化剂在生物环境中对单线态氧(~1O_2)的清除能力进行评价.测定了10个抗氧化剂的~1O_2清除能力和DPPH自由基清除能力,并从构效关系分析不同抗氧化剂~1O_2清除能力的不同.结果表明,该方法可以作为抗氧化剂~1O_2清除能力的一种常规实验室定量测定方法.

关 键 词:抗氧化活性  单线态氧(1O2)清除能力  评价  微生物纸片法  DPPH自由基

A biological method for the evaluation of the singlet oxygen(~1O_2) scavenging capacities of antioxidants
Institution:,Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission,Ministry of Education of China,Yunnan Minzu University,Yunnan University - based Key Laboratory of Clean Resource Conversion in Ethnic Regions,Yunnan Minzu University
Abstract:A biological method,named microbiological paper method,for the evaluation of singlet oxygen(1O2)scavenging capacities of antioxidants in biological systems was proposed. The1O2 scavenging capacities of 10 antioxidant compounds were determined. Furthermore,the results were also expounded reasonably by the structure-activity relation,and compared with those obtained by the DPPH · procedure. It was concluded that this assay could be utilized as a routine laboratory quantitative assay to evaluate the scavenging activity against1O2.
Keywords:antioxidant activity  singlet oxygen scavenging capacities  evaluation  microbiological paper method  DPPH·
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