三分类血液分析仪与血涂片镜检对白血病患者血液细胞分类结果差异分析

目的 探讨诊断白血病更准确、更全面的方法.方法 采用血液分析仪和手工涂片分类法同时诊断白血病.结果 14例ALL患者仪器不能进行白细胞分类和幼稚细胞提示;6例APL患者仪器进行了错误分类;24例AML患者仪器将血液中的幼稚细胞错误分类为小型白细胞;8例CML患者仪器将血液中的幼粒细胞及嗜酸嗜碱细胞分类为中间细胞群,以上均与手工分类有很大差异.结论 通过对56例白血病患者的血液分析,证实了在初诊白血病患者血样分析中,仪器不能给予白细胞分类及幼稚细胞的识别,对多形态、多变化性的血细胞识别有限.因此,用血液分析仪分析血样的同时,不能忽略血细胞形态学检查,防止幼稚细胞漏检.

Comparison of Three-classification Blood Cell Analyzer with Blood Smear for Hemocyte Classification of Leukemia

Objective To explore a more accurary and reliable method for leukemia diagnosis. Methods All patients were determined by blood cell analyzer and microscopic examination of the blood smear. Results The analyzer failed to classify leucocyte and recognize blast cell of fourteen patients; The analyzer made an erroneous classification of six APL patients; The analyzer classified blast cell in blood as LYM by mistaken of twenty-four patients; The analyzer classified premylocyte and basocyte and eosinocyte as mononuclcar, there were significant difference when comparison was made with classification of blood smear. Conclusion Through blood analysis for fifty-six leukemia patients, we have testified that the analyzer can not classify leucocyte and recognize blast cell and limite the recognization of polymorphic and diverse hemocyte during initial leukemia diagnosis with blood analysis. We can not neglect the examination of hemocyte morphology in order to prevent leak of blast cell when we analyzed blood sample with analyzer.