Analysis of gene expression patterns with cDNA microarray during late stage of spermatogenesis in mice |
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Authors: | Zuoren?Yu Jikui?Guan Yehua?Ge Jing?Ma Rui?Guo Sai?Li Shepu?Xue Email author" target="_blank">Daishu?HanEmail author |
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Institution: | (1) Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, 100005 Beijing, China |
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Abstract: | The differentiation process of round spermatids to spermatozoa during the late stage of spermatogenesis is called spermiogenesis. To explore spermiogenesis-related genes, cDNA microarray was used to study expression patterns of 1176 genes in pachytene spermatocytes, round spermatids and elongating spermatids of Balb/c mice. The results showed that 208 genes were detected in all the three cell types. Most of them were down-regulated from pachytene spermatocytes to round spermatids and elongating spermatids. However, up-regulation of 7 genes expression in round spermatids and 3 genes in elongating spermatids were found. Expression of 7 differentially expressed genes in cDNA arrays was further confirmed by semi-quantitative RT-PCR study. The RT-PCR results indicated that the expression of 6 genes was consistent with that in cDNA arrays, only one gene did not show differential expression by RT-PCR. These results may provide important clues for studying of expression, regulation, and function of spermiogenesis-related genes. |
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Keywords: | spermatogenesis spermiogenesis cDNA microarray gene expression pattern RT-PCR |
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