| 苞叶杜鹃离体培养及种质试管保存体系的建立 |
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| 引用本文： | 顾地周,邓志刚,綦茂伟,曹逊,朱俊义,姜云天.苞叶杜鹃离体培养及种质试管保存体系的建立[J].南京林业大学学报(自然科学版),2009,0(3):20-24. |
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| 作者姓名： | 顾地周 邓志刚 綦茂伟 曹逊 朱俊义 姜云天 |
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| 作者单位： | 1. 通化师范学院生物系,吉林,通化,134002
2. 通化市城市绿化管理处,吉林,通化,134001 |
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| 基金项目： | 吉林省科技厅资助项目，通化师范学院自然科学基金 |
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| 摘 要： | 以苞叶杜鹃新生嫩芽为外植体，应用均匀设计法筛选其最适合的培养基，建立了苞叶杜鹃的离体培养和种质试管保存体系。结果表明：适合苞叶杜鹃基部直接再生芽苗的诱导培养基为DR+TDZ(1.80 mg/L)+IBA(005 mg/L)，此条件下芽苗分化率为100%；生根培养基为MS(改良)+IBA(0.08 mg/L)+NAA(0.02 mg/L)+KT(0.15 mg/L)，这一条件下芽苗生根率达99%以上；试管保存培养基为1/7MS+B9(1.30 mg/L)+根皮苷(2.80 mg/L)，常温条件下保存时间超过35个月。以苞叶杜鹃再生植株茎节为材料进行快繁的结果表明，在35 d的培养周期内，平均增殖倍数超过55倍。
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| 关 键 词： | 苞叶杜鹃 离体快繁 试管保存 均匀设计 |
In vitro culture and in vitro germplasm preservation system of Rhododendron redowskianum Maxim. |
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| GU Di-zhou,DENG Zhi-gang,QI Mao-wei,CAO Xun,ZHU Jun-yi,JIANG Yun-tian.In vitro culture and in vitro germplasm preservation system of Rhododendron redowskianum Maxim.[J].Journal of Nanjing Forestry University(Natural Sciences ),2009,0(3):20-24. |
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| Authors: | GU Di-zhou DENG Zhi-gang QI Mao-wei CAO Xun ZHU Jun-yi JIANG Yun-tian |
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| Institution: | 1.Department of Biology, Tonghua Normal University, Tonghua 134002, China;
2.Tonghua City Afforestation Administrative Office, Tonghua 134001, China |
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| Abstract: | The tender buds of Rhododendron redowskianum Maxim.were used as explants and suitable medium compositions were screened through uniform design experiments.The results showed that DR+TDZ(1.80 mg/L)+IBA(0.05 mg/L) was the most suitable for shoots regeneration immediately at base of tender buds with a regeneration rate of 100%.MS(modified)+IBA(0.08 mg/L)+NAA(0.02 mg/L)+KT(0.15 mg/L) was most suitable for rooting with a rooting rate of 99%.1/7MS+B9(1.30 mg/L)+Phloridzin(2.80 mg/L) was most suitable for in vitro... |
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| Keywords: | Rhododendron redowskianum Maxim in vitro rapid propagation germplasm preservation uniform design |
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