腰带长体茧蜂卵和早期胚胎体外发育的初步研究

 通过在体外条件下培养腰带长体茧蜂侧输卵管处解剖得到的脱去滤泡细胞的成熟卵及从寄生早期幼虫血淋巴中获得的初期胚胎，对腰带长体茧蜂卵和胚胎的初期发育进行了初步的研究。结果表明，以TC-100为基础培养基加入一定量寄主幼虫血浆和胎牛血清时，从侧输卵管处解剖得到的卵在合适的体外培养条件下可以进行卵裂，且卵裂产生的初级胚胎可以被释放到培养基中。初级胚胎可以在胚外膜内进行分裂生成二级胚胎细胞，但二级胚胎不能被释放出胚外膜。从寄生初期寄主幼虫体内得到的初级胚胎可以在体外培养条件下长大并持续通过胚外膜凹陷进行增殖，但无法进入个体发育阶段，胚胎最后黑化死亡。

Preliminary Studies on the Development of Eggs and Early Embryos of Macrocentrus cingulum in vitro

The development of eggs and early embryos of Macrocentrus cingulum are preliminary studied by culturing the eggs without follicle cells dissected from wasps’ lateral oviduct and early embryos dissected from parasitized host larvae hemolymph in vitro. The results show that eggs dissected from the lateral oviducts may cleave in the culture medium composed of TC-100, plasma of larvae of its host Ostrinia furnacalis and fetal serum. Primary embryos are released from eggshell into the medium and subsequently cleaved into the secondary embryos. However, the secondary embryos within extraembryonic membrane can not be released. Early embryos dissected from host larvae hemocoel grow bigger in size and proliferate by the invagination of extroembryonic membrane, but they can not develop into the individual stage. Embryos cultured in vitro are finally dead in melanization.