| 杜氏藻与大肠杆菌融合初探 |
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| 引用本文： | 刘广发,楼士林.杜氏藻与大肠杆菌融合初探[J].厦门大学学报(自然科学版),1992,31(5):572-574. |
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| 作者姓名： | 刘广发 楼士林 |
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| 作者单位： | 厦门大学生物学系
(刘广发，楼士林，赖瑞卿)，厦门大学生物学系(李庆顺) |
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| 摘 要： | 杜氏藻(Dunaliella peircei Nicolai)系真核单胞绿藻,无纤维素细胞壁,细胞膜外由糖蛋白类物质组成柔软的包被,E.coli(Cm~r)内含带Cm~r基因的质粒。以对氯霉素(Cm)抗性的变化作为杜氏藻和大肠杆菌融合的选择标记,因而首先测定了它们在各自琼脂培养基上对Cm(原始浓度125g/1)的抗性水平(Tab.1)。
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| 关 键 词： | 杜氏藻 大肠杆菌 细胞融合 |
A Preliminary Research on Protoplast Fusion between Green Alga Dunaliella peircei and E. coli |
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| Liu Guangfa Lou Shilin Lai Ruiqing Li Qingshun.A Preliminary Research on Protoplast Fusion between Green Alga Dunaliella peircei and E. coli[J].Journal of Xiamen University(Natural Science),1992,31(5):572-574. |
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| Authors: | Liu Guangfa Lou Shilin Lai Ruiqing Li Qingshun |
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| Institution: | Dept. of Biol. |
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| Abstract: | The protoplasts of D, peircei (Chlorophyceae, Vohocales) can be obtained by inducing with pronase digestion. They were fused with the spheroplasts of E. Coli(Cmr) by polyethylene glycol(PEG) method. Some Dunaliella-like colonies were isolated from the selection media,with the chloramphenicol(Cm) resistibility rising from 70 ppm to 150 ppm. E. coli(Cmx) could still be found in the cells of fusants two weeks after fusion. |
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| Keywords: | Dunaltella peircei E coli(Cmx) Protoplast Fusion |
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