石防风胚性愈伤组织的诱导和体细胞胚的发生

石防风幼苗的根在含0.1-50mg/L 2,4-D的 MS 培养基中能诱导产生愈伤组织,最适浓度为0.5mg/L.愈伤组织来源于中柱内部的细胞。在0.5mg/L2,4-D 存在下,细胞分裂生长并发育至胚性细胞团或原胚,转移至无激素培养基后,愈伤组织表面及内部分化出发育阶段不同的体细胞胚,最后发育成为完整植株。在初生幼苗的胚轴上(有时在根或叶)常有次级胚的发生,它们能继续长成次级苗,并在苗上再次长胚,如此不断,直至多次。由此可见石防风体细胞胚发生的能力,是十分强大的。

EMBRYOGENIC CALLUSE INDUCTION AND SOMATIC EMBRYOGENESIS OF PEUCEDANUM TEREBINTHACEUM

Embryogenic calli could be obtained from the seedling root of Peuce danum terebinthaceum(Fiseh)Fisch,ex Turcz.(A chinese medicinal herb) when cultured on MS medium containing 0.1-50mg/1 2,4-D.The optimum concentration was 0.5mg/1. The callus originated from the parenchyma cells of the stele.When tranasferred to a medium without 2,4-D,embryoids differentiated on the callus and developed into plantlets.Histological observation of the diffe- rentiating callus showed embryos in different developing stages formed on the surface and also in the deep of callus.