Fine mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker |
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Authors: | Wenming Wang Yongli Zhou Guanhuai Jiang Bojun Ma Xuewei Chen Qi Zhang Lihuang Zhu Wenxue Zhai |
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Institution: | (1) Plant Biotechnology Laboratory, Chinese Academy of Sciences, 100101 Beijing, China;(2) Crop Institute, Chinese Academy of Agricultural Sciences, 100081 Beijing, China |
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Abstract: | An F2 population developed from theXa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene,Xa-4. Some restriction fragment length polymorphism (RFLP) markers on the high-density map constructed by Harushima et al. and
the amplified DNA fragments homologous to the conserved domains of plant disease resistance (R) genes were used to construct
the genetic linkage map around the geneXa-4 by scoring susceptible individuals in the population.Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction (PCR) marker M55. The R gene homologous fragment
marker RS13 was found co-segregating withXa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker
RS13 can be applied to molecular marker-assisted selection ofXa-4 in rice breeding programs. |
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Keywords: | rice bacterial blight resistance geneXu-4 genetic map molecular marker |
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