| 碳氮比对隐甲藻(Crypthecodinium cohnii)总脂及DHA含量的影响 |
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| 引用本文： | 王菊芳,梁世中,吴振强,谭字榴,陈峰.碳氮比对隐甲藻(Crypthecodinium cohnii)总脂及DHA含量的影响[J].华南理工大学学报(自然科学版),2000,28(10):28-31. |
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| 作者姓名： | 王菊芳 梁世中 吴振强 谭字榴 陈峰 |
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| 作者单位： | 1. 华南理工大学,生物科学与工程研究中心,广东,广州,510640
2. 香港大学,植物学系,香港 |
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| 基金项目： | 广东省博士启动基金，970486， |
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| 摘 要： | 研究了碳氮比对隐甲藻（Ｃｒｙｐｔｈｅｃｏｄｉｎｉｕｍ ｃｏｈｎｉｉ ＡＴＣＣ ３０５５６）产脂及ＤＨＡ含量的影响。结果表明：在碳氮比为６～３１的研究范围内，随着碳氮比的提高，隐甲藻最大生物量呈现下降趋势，ＤＨＡ占总脂肪酸的比率降低，而隐甲藻的脂质含量在碳氮比为２１时出现低值临界点。以收获ＤＨＡ为目标的隐甲藻培养的最佳碳氮比为１１。在此条件下，隐甲藻培养５６ｈ可以获得生物量１１．８４ｇ／Ｌ（干重），
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| 关 键 词： | 碳氮比 隐甲藻 DHA 脂质含量 |
| 修稿时间： | 1999-10-25 |
Effects of C/N Ratio on the Content of Lipids and DHA by Crypthecodinium cohnii |
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| WANG Ju-fang,LIANG Shi-zhong,WU Zhen-qiang,TAN Zi-liu,CHEN Feng.Effects of C/N Ratio on the Content of Lipids and DHA by Crypthecodinium cohnii[J].Journal of South China University of Technology(Natural Science Edition),2000,28(10):28-31. |
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| Authors: | WANG Ju-fang LIANG Shi-zhong WU Zhen-qiang TAN Zi-liu CHEN Feng |
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| Abstract: | Culture of Crypthecodinium cohnii is a potential alternative approach to produce DHA for the demand of medical uses. The effect of the carbon to nitrogen (C/N) ratio of the medium on the content of lipids and Docosahexaenoic acid (DHA) of Crypthecodinium cohnii ATCC 30556 was investigated. At the range of 6 to 31, with the ratio of C/N increasing, both the maximal biomass and the DHA to total fatty acids ratio decreased. A C/N ratio of approximately 21 was regarded as low critical value in the culture of Crypthecodinium cohnii ATCC 30556. The optimized C/N ratio was 11; at this value, the productivity of biomass reached 11. 84 g DW/L and the yield of DHA achieved 612. 13 mg/L after 56 hours. |
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| Keywords: | C/N ratio Crypthecodinium cohnii DHA lipid |
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