金莲花中荭草苷在兔体内组织中的分布特征研究

建立HPLC法测定兔组织中荭草苷含量的方法,研究静脉给药时,荭草苷在新西兰白兔体内组织中的分布特点,荭草苷给药后,分时采集组织样品,放血处死动物,取出心、肝、脑、肾、肺、脾组织并制备匀浆,以HPLC法测定兔组织中荭草苷含量,采用统计矩理论计算各个组织的AUC。组织中的内源性物质不干扰荭草苷的测定,在0.050 0～50.0mg/L范围内,荭草苷质量浓度和峰面积线性关系良好,定量下限为0.050 0mg/L。精密度、准确度和加样回收率RSD值均小于15%。各组织的AUC顺序为肾>肝>肺>脾>心>脑。将各组织AUC进行对数转换后,依次进行正态性检验、方差齐性检验和方差分析,结果显示,不同组织的AUC有显著性差异(p<0.05),荭草苷在新西兰白兔体内主要组织中的含量分布有明显不同。

Distribution of orientin from Trollius Chinensis in tissue of rabbits

A method using HPLC is established to determine orientin in tissue of rabbits and to study the characteristic of the orientin when injected into veins. After orientin is injected into veins,tissue samples are collected at time sharing.The rabbit is put to death by bloodletting,then the heart,liver,brain,kidney,lung and spleen are taken out to prepare homogenate tissue.HPLC is used for determination of orientin in tissue of rabbits.Theory of statistical moment is used to account AUC of each tissue sample.Endogenous material in tissue does not interfere in determination of orientin.In amplitude of 0.050 0～50.0 mg/L,mass concentration of orientin and peak area have a good linear relation,and the quantitation of lower limit is 0.050 0 mg/L.The RSD of precision,accuracy and plus sample recovery are all less than 15%.The AUC order of each tissue is kidney>liver>lung>spleen>heart>brian.After log transition of AUC data,normality test,homogeneity test for variance and analysis of variance are conducted.The result shows AUC of each tissue have significant difference(p<0.05).Orientin distribute in dominating tissue of rabbits is obviously different.