Hormone and forskolin-stimulated cyclic AMP accumulation in human lymphocytes: reliability of longitudinal time measurements |
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| Authors: | R P Ebstein J Mintzer Y Lipschitz Z Shemesh J Stessman |
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| Institution: | (1) Jerusalem Mental Health Center-Ezrath Nashim, P.O.B. 140, Jerusalem, (Israel) |
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| Abstract: | Summary Reliability of measurement of lymphocyte cyclic AMP synthesis in intact cells was estimated by taking 3 successive blood samples during a one-month period from 11 healthy volunteers. Isoproterenol and prostaglandin E1-stimulated cyclic AMP accumulation were used to evaluate the activity of these two receptor activities in human lymphocytes. Forskolin-stimulated cyclic AMP accumulation was used to evaluate the activity of the Ns/catalytic subunit. Only for forskolin was significant reliability observed. For isoproterenol and prostaglandin E1 significant reliability was observed only for male subjects.Acknowledgment. This work was supported in part by the following organizations: Chief Scientist's Office, Israel Ministry of Health; Herman Goldman Foundation, New York; United Jewish Endowment Fund of Greater Washington-Pollinger Foundation. |
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| Keywords: | Cyclic AMP synthesis measurement in lymphocytes forskolin isoproterenol prostaglandin E1 |
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