南水北调中线工程水源区抗生素抗性基因多样性研究

为了研究丹江口水库和汉江抗生素抗性基因(Antibiotic Resistance Genes, ARGs)的污染现状与分布规律, 采用高通量测序的方法, 于2014年春季和秋季分别对丹江口水库及汉江沿程的ARGs多样性进行检测。分别在春秋两季样本中检测出21和19类ARGs, 其中9类ARGs是水体ARGs的主要组成部分。杆菌肽类ARGs是水体中最主要的ARGs, 而β-内酰胺类ARGs在春季丹江口水库陶岔采样点中占比最高。秋季水体各采样点的ARGs组成结构差异比春季小, 通过NMDS和ANOSIM分析发现ARGs的组成存在显著的季节差异, 甲氧苄氨嘧啶类、多粘菌素类和多重耐药类ARGs是具有显著季节差异的ARGs种类。由相关性分析发现13类具有互相显著强相关关系的ARGs, 其中相关性最强的ARGs很可能共存于一种微生物中。此外, 四环素类和氨基糖苷类ARGs可能作为预测水源区中与其共存ARGs相对丰度的指示种类。研究结果可为饮用水源区的水质保护和ARGs 污染防治与管理提供科学依据。

Diversity of Antibiotic Resistance Genes in Water Source Areas of the Middle Route of the South-to-North Water Transfer Project

In order to study the pollution status and regularity of antibiotic resistance genes (ARGs), highthroughput sequencing methods were used to analyze the diversity of ARGs along Danjiangkou Reservoir and Hanjiang River in spring and autumn of 2014. 21 and 19 types of ARGs were detected in spring and autumn samples respectively. Among them, 9 types of ARGs were the main types of ARGs content. Bacitracin was the most important ARGs in water, while β-lactam accounted for the highest proportion in the Taocha sampling sites of Danjiangkou Reservoir. The composition difference of ARGs among sampling sites in Water_Autumn samples was smaller than that in spring. NMDS and ANOSIM analysis showed significant seasonal differences in the composition of ARGs. Trimethoprim, polymyxin and multidrug were ARGs which had significant seasonal differences. Correlation analysis found 13 types of ARGs with significantly strong correlations with each other, and the most relevant ARGs were likely to coexist in the same microorganism. In addition, tetracycline and aminoglycoside may be useful as indicators for predicting the relative abundance of ARGs coexisting in the water source region. This study could provide reference for water quality protection and ARGs pollution prevention and management in drinking water source areas.