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忍冬藤可溶性膳食纤维提取及性能分析
引用本文:忍冬藤可溶性膳食纤维提取及性能分析.忍冬藤可溶性膳食纤维提取及性能分析[J].山东科学,2017,30(1):20-25.
作者姓名:忍冬藤可溶性膳食纤维提取及性能分析
作者单位:山东中医药大学药学院,山东 济南 250355
基金项目:山东省科技发展计划(2014GSF11903);2015年度大学生研究训练计划(SRT)(201510441023)
摘    要:探讨纤维素酶法提取忍冬藤中水溶性纤维素的最佳工艺并对其性能进行分析。在单因素试验基础上,采用正交试验对提取工艺进行优化得到最佳工艺条件组合为p H=4.8,温度45℃,料液比1:12(g/m L),纤维素酶体积分数1.2%,提取时间6 h,浸提3次。在此条件下,水溶性膳食纤维的提取率为3.57%,所提取的水溶性纤维素持水力为6.1 g/g,膨胀力为5.3 m L/g,持油力为4.53 g/g,阳离子交换能力为0.58 mmol/g,DPPH自由基最大清除率为80.13%,具有较好的理化性能。研究认为,纤维素酶解法可用于提取忍冬藤中的水溶性膳食纤维。

关 键 词:水溶性膳食纤维  忍冬藤  纤维素酶  性能  
收稿时间:2016-04-26

Extraction and property analysis of soluble dietaryfiber from stems of Lonicera japonica
GUO Feng-qian,HUANG Zu-shun,ZHU Bao-lei,LIU Qian.Extraction and property analysis of soluble dietaryfiber from stems of Lonicera japonica[J].Shandong Science,2017,30(1):20-25.
Authors:GUO Feng-qian  HUANG Zu-shun  ZHU Bao-lei  LIU Qian
Institution:Shandong University of Traditional Chinese Medicine, Jinan 250355, China
Abstract:The optimal technology of extracting soluble dietary fiber (SDF) from stems of Lonicera japonica by cellulose method was investigated and the properties of SDF were also analyzed. Based on single factor experiments, orthogonal experiments were performed to optimize the extractive conditions, and the results showed the optimum conditions were pH 4.8, at 45 ℃, 1∶12(g/mL)of material and water, cellulase 1.2% by volume, extracting for 6 h, 3 times.Under the above conditions, the yield of SDF was 3.57%, whose water holding capacity was 6.1 g/g, expansion force was 5.3 mL/g, oil holding capacity was 4.53 g/g, cation exchange capacity was 0.58 mmol/g, highest free radical scavenging rate on DPPH was 80.13%, and the SDF had excellent physical and chemical properties. The study indicated that the cellulose method can be used to extracting SDF from stems of Lonicera japonica.
Keywords:stems of Lonicera japonica  cellulase  water soluble dietary fiber  properties  
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