| 胰蛋白酶水解脱脂豆粕的研究 |
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| 引用本文： | 黄丹.胰蛋白酶水解脱脂豆粕的研究[J].四川理工学院学报(自然科学版),2004,17(2):46-48. |
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| 作者姓名： | 黄丹 |
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| 作者单位： | 四川理工学院生物工程系,四川,自贡,643000 |
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| 摘 要： | 以脱脂豆粕为底物,通过正交实验和水解蛋白曲线的分析,确定了用稀酸处理脱脂豆粕的最佳酸提条件以及用胰蛋白酶酶解脱脂豆粕的最佳酶解条件。最佳酸提条件为温度60℃,时间5h,固液比4%。最佳酶解条件为酶解温度40℃,酶-底物浓度比1.5%,底物浓度5%,pH9.0,时间15h。
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| 关 键 词： | 胰蛋白酶 脱脂豆粕 酶解条件 |
| 文章编号： | 1008-438X(2004)02-0046-03 |
| 修稿时间： | 2004年3月30日 |
Study of the Hydrolysis of the Degreased Soja Draft by Trypsin |
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| HUANG Dan.Study of the Hydrolysis of the Degreased Soja Draft by Trypsin[J].Journal of Sichuan University of Science & Engineering:Natural Science Editton,2004,17(2):46-48. |
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| Authors: | HUANG Dan |
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| Abstract: | Using degreased soja draffs as the substrate, the best conditions of the acerbic abstract and best conditions of the hydrolysis of the degreased soja draff are found by orthogonal test making out the curve of hydrolyzing proteins. In experiments, an optimum acerbic abstract condition are temperature 60℃,duration 5h, proportion of solid and liquid 4%,optimum hydrolyzing conditions are temperature 40℃,enzyme/substrate1.5%,substrate concen 5%,pH9.0 and duration 15h. |
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| Keywords: | trypsin the degreased soja draff enzyme hydralysing conditions |
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