不同年龄大鼠双核肝细胞变化的研究

胶原酶灌注法、固定液灌注法及针头法用于肝细胞的分离效果良好。本文根据大鼠的不同年龄和实验需要,分别采用以上三种方法,对出生后4天到两年以上的12个年龄组的大鼠进行了双核肝细胞数统计,并与切片法做了对照。双核肝细胞随大鼠的年龄变化很大。出生后不久,只有一个很低的值,20天后开始迅速增加,40天左右达到最大值(45％),成年期降至25％,其后,直到老年基本趋于稳定状态。切片法获得的双核百分率只有灌注法的1/3到1/2,说明用切片法统计双核数是不准确的。双核百分率与生态学上的其他指标结合起来可以推测大鼠的年龄。

STUDY ON THE CHANGES OF BINUCLEATE HEPATOCYTES OF RATS AT DIFFERENT AGES

The collagenase perfusion, the fixative perfusion and the needle methods are effective in dissociating hepatic cells. In this report, in accordance with the different ages of rats as well as the need for the experiment, the above methods are adopted respectively to count the binucleate cells in rat liver. The age of rats ranges from the 4th day after birth to about over two years. A contrast experiment has been made with the section method. It was found that the relative number of binucleate cells in the rat liver varied significantly with the age of the animal. Shortly after birth, there were very few binucleate cells and there was little change until about 20 days when the incidence rose abruptly, and reached the highest value(45%)after 40 days. It declined mored slowly to about 25%in adult. Then, the percentage of binucleate hapatic cells was permanent. The percentage obtained with the section method was only about half to one-third of the perfusion method. These results indicate that the counting of the binucleate cells with the section method is not accurate, but the precentage of the binucleate cells together with other quota in ecology can predict the age of rats.