| 维生素D受体蛋白及其mRNA在人输卵管黏膜上皮的表达 |
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| 引用本文： | 张纯,朱伟杰,徐建平.维生素D受体蛋白及其mRNA在人输卵管黏膜上皮的表达[J].暨南大学学报,2009,30(2). |
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| 作者姓名： | 张纯 朱伟杰 徐建平 |
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| 作者单位： | 1. 暨南大学生命科学技术学院,生殖免疫研究所,广东,广州,510632
2. 暨南大学附属第一医院,妇产科,广东,广州,510630 |
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| 摘 要： | 目的:探讨人输卵管黏膜上皮维生素D受体(VDR)蛋白及其mRNA的表达.方法:30例输卵管标本取自具有正常妊娠生育史,因子宫肌瘤、子宫腺肌病等行腹式子宫切除术,一并切除输卵管的妇女.输卵管取材包括峡部、壶腹部和伞部,按子宫内膜组织学分期分为增生早期组6例、增生中晚期组9例、分泌早期组7例、分泌中晚期组8例.应用免疫组织化学法和逆转录聚合酶链反应法检测人输卵管黏膜上皮VDR的表达.结果:人输卵管黏膜上皮中检测到VDR蛋白及其mRNA表达.VDR蛋白主要表达于输卵管上皮细胞的细胞核中,部分间质平滑肌细胞核内亦有表达.相同时期各不同部位输卵管上皮细胞VDR蛋白表达无明显差别(P＞0.05),随月经周期变化,各不同部位输卵管上皮细胞VDB蛋白表达发生改变,在增生早期和分泌中晚期较低,与之比较,在增生中晚期和分泌早期其表达明显增高(P＜0.01),至分泌早期达最高值;壶腹部输卵管黏膜上皮组织VDR mRNA表达在增生早期和分泌中晚期较低,在增生中晚期和分泌早期明显增高(P＜0.01).结论:人输卵管黏膜上皮组织存在VDR蛋白及其mRNA表达,在增生中晚期和分泌早期其表达明显增高,且具有周期性变化.
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| 关 键 词： | 维生素D受体 人输卵管 免疫组织化学 逆转录聚合酶链反应 |
Expressions of the vitamin D receptor protein and its mRNA in mucosa epithelium of human fallopian tubes |
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| ZHANG Chun,ZHU Wei-jie,XU Jian-ping.Expressions of the vitamin D receptor protein and its mRNA in mucosa epithelium of human fallopian tubes[J].Journal of Jinan University(Natural Science & Medicine Edition),2009,30(2). |
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| Authors: | ZHANG Chun ZHU Wei-jie XU Jian-ping |
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| Affiliation: | 1.Institute of Reproductive Immunology;College of Life Science and Technology;Jinan University;Guangzhou 510632;China;2.Department of Obstetrics and Gynecology;the First Affiliated Hospital;Guangzhou 510630;China |
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| Abstract: | Aim:To investigate the expressions of vitamin D receptor(VDR) protein and its mRNA in mucosa epithelium of human fallopian tubes. Methods: Samples of fallopian tubes were obtained from 30 women undergoing abdominal hysterectomy with adnexectomy for benign disease such as uterine leiomyoma,adenomyosis in the pelvic cavity.The isthmic,ampullary and fimbrial portions of fallopian tubes were respectively collected.These specimens were divided into 4 groups based on their endometrium histological staging: early-... |
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