| 不同分子质量软骨多糖对K562细胞的增殖抑制作用 |
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| 引用本文： | 宋微,刘安军,王稳航,刘玉江.不同分子质量软骨多糖对K562细胞的增殖抑制作用[J].天津科技大学学报,2006,21(3):20-22. |
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| 作者姓名： | 宋微 刘安军 王稳航 刘玉江 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津,300457 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 研究不同分子质量的软骨多糖对慢性髓系红白血病K562细胞的增殖抑制作用。用不同浓度的酒精对软骨提取物进行分级沉淀，得到不同分子质量的软骨多糖，并用聚丙烯酰胺凝胶电泳（SDS-PAGE）检测所得软骨多糖的纯度及分子质量；采用噻唑蓝（MTF）比色法测定细胞的增殖活性；用琼脂糖凝胶电泳法检测DNA电泳特征。结果显示，短链软骨多糖（分子质量约为30ku）对K562细胞具有明显的增殖抑制作用，这种抑制作用呈现明显的时间依赖性，且琼脂糖凝胶电泳出现明显的DNA梯状条带。
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| 关 键 词： | 软骨多糖 K562细胞 MTT法 DNA电泳 |
| 文章编号： | 1672-6510(2006)03-0020-03 |
| 修稿时间： | 2005年11月7日 |
Inhibition of K562 Cell Proliferation by Cartilage Polysaccharide with Different Molecular Weight |
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| SONG Wei,LIU An-jun,WANG Wen-hang,LIU Yu-jiang.Inhibition of K562 Cell Proliferation by Cartilage Polysaccharide with Different Molecular Weight[J].Journal of Tianjin University of Science & Technology,2006,21(3):20-22. |
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| Authors: | SONG Wei LIU An-jun WANG Wen-hang LIU Yu-jiang |
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| Abstract: | The aim of this paper was to study the effect of the proliferation of chronic myegenous leukemia ( K562) treated by different molecular weight cartilage polysaccharide( sc-PS). Cartilage extract was precipitated by ethanol with different concentration. Different molecular weight polysaccharide was collected. Then concentration and molecular weight of them were studied by SDS-PAGE; MTT method was used to test the proliferation of cells treated by sc-PS; Agarose gel electro-phoresis was used to detect the characteristic of DNA; The results indicated that the growth of K562 cells was inhibited obviously by sc-PS, which in a dose-dependent, DNA ladder could be observed apparently in agarose gel electrophoresis. |
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| Keywords: | polysaccharide K562 cell MTT method DNA agarose gel electrophoresi |
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