| 利用透明颤菌血红蛋白基因在谷氨酸棒杆菌中的表达提高谷氨酸和谷氨酰胺的产量 |
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| 引用本文： | 孙智杰,梁楠,李润东,沈忠耀,陈国强.利用透明颤菌血红蛋白基因在谷氨酸棒杆菌中的表达提高谷氨酸和谷氨酰胺的产量[J].北京理工大学学报,2005,25(2):180-184. |
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| 作者姓名： | 孙智杰 梁楠 李润东 沈忠耀 陈国强 |
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| 作者单位： | 北京理工大学生命科学与技术学院,北京,100081;清华大学生物科学与技术系,北京,100084;清华大学化工系,北京,100084 |
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| 摘 要： | 为了降低谷氨酰胺(glutamine,Gln)生产过程中所需要的高溶氧水平对设备的要求,在谷氨酸棒杆菌C.glutamicum ATCC 14067中表达增强摄氧的透明颤菌血红蛋白基因(Vitreoscilla hemoglobin gene,vgb),以此提高细胞的摄氧能力及能量的供给水平.对得到的重组菌和野生菌进行了发酵实验.在溶氧只有5%的条件下,重组菌比野生菌细胞干重提高了1.2倍,单位细胞谷氨酸的生产能力提高了6.5倍,单位细胞谷氨酰胺的生产能力提高了1.4倍.对比结果显示,含有vgb基因的重组菌比野生菌在细胞生长、谷氨酸和谷氨酰胺合成等方面的能力都有显著提高.
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| 关 键 词： | 透明颤菌血红蛋白基因 谷氨酸棒杆菌 谷氨酸 谷氨酰胺 发酵 |
| 文章编号： | 1001-0645(2005)02-0180-05 |
| 收稿时间： | 2004/5/22 0:00:00 |
| 修稿时间： | 2004年5月22日 |
Improving Production of Glutamic Acid and Glutamine Using Recombinant Corynebacterium glutamicum Harboring Hemoglobin Gene from Vitreoscilla sp. |
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| SUN Zhi-jie,LIANG Nan,LI Run-dong,SHEN Zhong-yao and CHEN Guo-qiang.Improving Production of Glutamic Acid and Glutamine Using Recombinant Corynebacterium glutamicum Harboring Hemoglobin Gene from Vitreoscilla sp.[J].Journal of Beijing Institute of Technology(Natural Science Edition),2005,25(2):180-184. |
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| Authors: | SUN Zhi-jie LIANG Nan LI Run-dong SHEN Zhong-yao and CHEN Guo-qiang |
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| Institution: | School of Life Science and Technology, Beijing Institute of Technology, Beijing100081, China;School of Life Science and Technology, Beijing Institute of Technology, Beijing100081, China;Department of Biological Sciences and Biotechnology,Tsinghua University, Beijing100084, China;Department of Chemical Engineering, Tsinghua University, Beijing100084, China;Department of Biological Sciences and Biotechnology,Tsinghua University, Beijing100084, China |
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| Abstract: | The expression of Vitreoscilla hemoglobin gene (vgb gene) in Corynebacterium glutamicum ATCC 14067 increased the oxygen and energy supply resulting in improving glutamic acid (Glu) and glutamine (Gln) productivity. Under 5% dissolved oxygen, the cell dry weight (CDW) of the recombinant strain and wild strain were 45.1 g/L and 54.1 g/L respectively, the concentration of Glu were 9.56 g/L and 1.27 g/L, the concentration of Gln were 5.51 g/L and 2.27 g/L. The results indicated that increasing oxygen supply via vgb gene expressing in recombinant C. glutamicum remarkably promote cell growth and production of Glu and Gln. This could be an effective way to reduce the cost of industrial fermentation of Glu and Gln. |
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| Keywords: | Vitreoscilla hemoglobin gene Corynebacterium glutamicum glutamic acid glutamine fermentation |
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